Two new 4-Cys conotoxins (framework 14) of the vermivorous snail Conus austini from the Gulf of Mexico with activity in the central nervous system of mice.

Peptides
Alejandro Zugasti-CruzEdgar P Heimer de la Cotera

Abstract

As part of continuing studies of the venom components present in Conus austini (syn.: Conus cancellatus), a vermivorous cone snail collected in the western Gulf of Mexico, Mexico, two major peptides, as14a and as14b, were purified and characterized. Their amino acid sequences were determined by automatic Edman sequencing after reduction and alkylation. Their molecular masses, established by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, confirmed the chemical analyses and indicated that as14a and as14b have free C-termini. Each peptide contains 4-Cys residues arranged in a pattern (C-C-C-C, framework 14). The primary structure of as14a is GGVGRCIYNCMNSGGGLNFIQCKTMCY (experimental monoisotopic mass 2883.92Da; calculated monoisotopic mass 2884.20Da), whereas that of as14b is RWDVDQCIYYCLNGVVGYSYTECQTMCT (experimental monoisotopic mass 3308.63Da; calculated monoisotopic mass 3308.34Da). Both purified peptides elicited scratching and grooming activity in mice, and as14b also caused body and rear limb extension and tail curling immediately upon injection. The high sequence similarity of peptide as14a with peptide vil14a from the vermivorous C. villepinii suggests that the former might block K+ channels.

References

Jan 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·V Erspamer
Jul 20, 1990·Science·B M OliveraL J Cruz
Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·C ClarkL J Cruz
Jul 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·D B NielsenJ M McIntosh
Dec 23, 1999·Current Opinion in Neurobiology·B M OliveraD Yoshikami
Feb 26, 2000·Biochemistry·M B LirazanB M Olivera
May 5, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P A Anderson, R M Greenberg
Nov 2, 2001·Biochemical and Biophysical Research Communications·G R MassiliaF Polticelli
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·M MailloE P Heimer de la Cotera
Feb 5, 2002·Peptides·D A KeireJ R Reeve
Jul 16, 2002·The Journal of Biological Chemistry·Ryo FujiiMasahiko Fujino
Apr 16, 2003·Journal of Neurochemistry·Elsie C JimenezBaldomero M Olivera
Jul 29, 2004·Current Medicinal Chemistry·Bruce G LivettZeinab Khalil
Aug 24, 2004·Current Protein & Peptide Science·M A GrantA C Rigby
Dec 28, 2004·Molecular Phylogenetics and Evolution·Thomas F Duda, Alan J Kohn
Jan 13, 2005·Molecular Ecology·Thomas F Duda, Emilio Rolán
Dec 8, 2005·Biochemistry·Carolina MöllerFrank Marí
Sep 6, 2006·Toxicon : Official Journal of the International Society on Toxinology·Raymond S Norton, Baldomero M Olivera
Aug 1, 1992·The Biological Bulletin·L J CruzB M Olivera

❮ Previous
Next ❯

Citations

Jun 21, 2013·Marine Drugs·Jorge NevesVitor Vasconcelos
Dec 19, 2014·Marine Drugs·Samuel D Robinson, Raymond S Norton
Aug 10, 2010·Peptides·Can PengChengwu Chi
May 31, 2015·Genome Biology and Evolution·Juliette GorsonMandë Holford
May 25, 2015·Asian Pacific Journal of Tropical Medicine·Palanisamy Satheesh KumarSundaresan Umamaheswari
Apr 6, 2018·Marine Drugs·Ashlin H TurnerChristina I Schroeder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.