Two New Lytic Bacteriophages of the Myoviridae Family Against Carbapenem-Resistant Acinetobacter baumannii

Frontiers in Microbiology
Weilong ZhouZhiyong Zong

Abstract

Two lytic bacteriophages, WCHABP1 and WCHABP12, were recovered from hospital sewage and were able to infect 9 and 12 out of 18 carbapenem-resistant Acinetobacter baumannii clinical strains, which belonged to different clones. Electron microscopy scan showed that both bacteriophages had the similar morphology as those of the Myoviridae family. Whole genomic sequencing revealed 45.4- or 45.8-kb genome with a 37.6% GC content for WCHABP1 and WCHABP12, both of which showed significant DNA sequence similarity with bacteriophages of the Ap22virus genus within the Myoviridae family. Taxonomic analysis was therefore performed following the proposal approved by the International Committee on Taxonomy of Viruses, which confirmed that WCHABP1 and WCHABP12 represented two new species of the Ap22virus genus. No tRNAs but 88 and 89 open reading frames (ORFs) were predicted for the two bacteriophages, among which 22 and 21 had known function and encoded proteins for morphogenesis, packaging, lysis, and nucleiotide metabolism. The C-terminal amino acids of the large unit of fiber tail proteins varied between the bacteriophages, which may explain their different host ranges. For most lytic bacteriophages, a set of holin and endolysin are requir...Continue Reading

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Citations

Dec 18, 2019·Current Pharmaceutical Biotechnology·Niran Adhikari, Krishna Prasad Acharya
Nov 9, 2018·Frontiers in Cellular and Infection Microbiology·Lucy L FurfaroBarbara J Chang
Apr 17, 2019·Frontiers in Microbiology·Mansura S MulaniKarishma R Pardesi
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Nov 18, 2020·International Journal of Molecular Sciences·Corneliu Ovidiu VrancianuMariana Carmen Chifiriuc
May 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Zhenhua HuangYong Zhao
Jul 3, 2021·Antibiotics·Antonio Broncano-LavadoMeritxell García-Quintanilla
Jul 2, 2021·Environmental Microbiology Reports·Sandhya Rai, Amod Kumar

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Methods Mentioned

BETA
bronchoalveolar lavage
electrophoresis
electron microscopy

Software Mentioned

SE
tRNAscan
BLASTp
Prokka
HMMER
PhyML
BLAST Ring Image Generator ( BRIG )
SPAdes
MUSCLE
GraphPad Prism

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