Two novel HLA-A*0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice.

Veterinary Research
Ying-Kit CheungYong Xie

Abstract

The influenza A nucleoprotein (NP) is an attractive target for avian flu vaccine development because of its high conversancy in the evolutionary chain of the virus. Here we identified two novel HLA-A*0201 restricted NP epitopes, named H5N1 NP373-381 AMDSNTLEL (NP373) and NP458-466 FQGRGVFEL (NP458), using computational bioinformatic analysis. The NP peptides showed a high binding affinity to HLA-A*0201 on T2 cells, and were able to induce the activation of the cytotoxic T cells in the human peripheral blood mononuclear cells. We examined the potential of using NP373 and NP458 peptide sequences supplemented with a single-chain trimer as potential DNA vaccine candidates in an HHD transgenic mouse model. A gene gun delivery system was used for administrating the vaccine candidates into the animals. The results from cytotoxicity and ELISPOT assays indicated that a significant amount of IFN-gamma was secreted by the T cells of the vaccinated mice, and the T cells were able to eliminate the corresponding peptide-loaded T2 cells. The discovery of these novel immunogenic NP peptides provides valuable information for avian flu vaccine design and construction.

References

Apr 18, 2000·The Journal of General Virology·Y HiromotoK Nerome
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yik Y L YuTed H Hansen
Jul 27, 2002·Emerging Infectious Diseases·Suzanne L EpsteinJacqueline M Katz
Dec 19, 2002·The Journal of Clinical Investigation·Valérie DutoitDanila Valmori
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sacha GnjaticLloyd J Old
May 20, 2003·Tissue Antigens·D MiddletonR Komerofsky
Dec 4, 2003·Lancet·Karl G NicholsonMaria Zambon
Sep 27, 2005·Immunological Reviews·Peter CresswellPamela A Wearsch
Oct 3, 2006·Trends in Molecular Medicine·Taisuke Horimoto, Yoshihiro Kawaoka
Feb 20, 2007·Vaccine·Dominick J LaddyDavid B Weiner
Jun 2, 2007·Medical Microbiology and Immunology·Jindrich CinatlHans W Doerr
Dec 18, 2007·Current Opinion in Biotechnology·Guus F RimmelzwaanAlbert D M E Osterhaus

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Citations

Apr 6, 2011·Immunotherapy·Lindzy F DodsonPeter Goedegebuure
Dec 5, 2013·Human Vaccines & Immunotherapeutics·Fazren AzmiIstvan Toth
Jul 10, 2018·Briefings in Functional Genomics·Kashyap Kumar DubeyPratyoosh Shukla
Jul 14, 2010·Expert Review of Vaccines·Sheeba Alam, Douglas G McNeel

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Methods Mentioned

BETA
transgenic
density gradient centrafugation
PCR
flow cytometry

Software Mentioned

SYFPEITHI

Related Concepts