Two Novel Hypovirulence-Associated Mycoviruses in the Phytopathogenic Fungus Botrytis cinerea : Molecular Characterization and Suppression of Infection Cushion Formation

Viruses
Fangmin HaoGuoqing Li

Abstract

Botrytis cinerea is a necrotrophic fungus causing disease on many important agricultural crops. Two novel mycoviruses, namely Botrytis cinerea hypovirus 1 (BcHV1) and Botrytis cinerea fusarivirus 1 (BcFV1), were fully sequenced. The genome of BcHV1 is 10,214 nt long excluding a poly-A tail and possesses one large open reading frame (ORF) encoding a polyprotein possessing several conserved domains including RNA-dependent RNA polymerase (RdRp), showing homology to hypovirus-encoded polyproteins. Phylogenetic analysis indicated that BcHV1 may belong to the proposed genus Betahypovirus in the viral family Hypoviridae. The genome of BcFV1 is 8411 nt in length excluding the poly A tail and theoretically processes two major ORFs, namely ORF1 and ORF2. The larger ORF1 encoded polypeptide contains protein domains of an RdRp and a viral helicase, whereas the function of smaller ORF2 remains unknown. The BcFV1 was phylogenetically clustered with other fusariviruses forming an independent branch, indicating BcFV1 was a member in Fusariviridae. Both BcHV1 and BcFV1 were capable of being transmitted horizontally through hyphal anastomosis. Infection by BcHV1 alone caused attenuated virulence without affecting mycelial growth, significantly i...Continue Reading

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Citations

Mar 9, 2019·Archives of Virology·Luis CottetAntonio Castillo
Apr 24, 2019·Viruses·Ioly Kotta-Loizou
Mar 28, 2019·Virus Genes·Antonio Charlys da CostaAdriana Luchs
Dec 24, 2019·Frontiers in Microbiology·Amélie de ValléeNathalie Poussereau
Feb 11, 2020·Frontiers in Microbiology·Almudena Torres-TrenasEncarnación Pérez-Artés
Jun 30, 2019·Archives of Virology·Jing WangHongyan Liu
Jun 4, 2019·Frontiers in Microbiology·Huan LiLiying Sun
Jul 1, 2020·Annual Review of Phytopathology·Maren L Friesen
Jun 11, 2019·Frontiers in Cellular and Infection Microbiology·Vaskar Thapa, Marilyn J Roossinck
Jan 23, 2020·International Journal of Molecular Sciences·Xinqiang LiuJiasen Cheng
Feb 5, 2021·Environmental Microbiology·Mathias ChoquerNathalie Poussereau
May 13, 2021·MBio·Ana Ruiz-PadillaMaría A Ayllón
Jun 15, 2021·Frontiers in Microbiology·Yan PengBing L Xu

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Methods Mentioned

BETA
electrophoresis
PCR
Infection

Software Mentioned

BlastP
BlastN
BlASTp search
BLAST
MUSCLE
TMHMM
CDD
MEGA
Hel

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