Two novel neutralizing antigenic epitopes of the s1 subunit protein of a QX-like avian infectious bronchitis virus strain Sczy3 as revealed using a phage display peptide library

Veterinary Immunology and Immunopathology
Nianli ZouYong Huang

Abstract

The spike (S) protein of the infectious bronchitis virus (IBV) plays a central role in the pathogenicity, the immune antibody production, serotype and the tissue tropism. In this study, we generate 11 monoclonal antibodies (mAbs) against S1 subunit of IBV Sczy3 strain, and two mAbs 1D5 and 6A12 were positive in indirect ELISA against both His-S1 protein and the purified whole viral antigen. MAb 6A12 and 1D5 could recognized by other 10 IBV strains (IBVs) from five different genotypes, except that 1D5 had a relatively low reaction with two of the 10 tested IBVs. End-point neutralizing assay performed in chicken embro kidney (CEK) cells revealed that the neutralization titer of 6A12 and 1D5 against Sczy3 reached 1:44.7 and 1:40.6, respectively. After screening a phage display peptide library and peptide scanning, we identified two linear B-cell epitopes that were recognized by the mAbs 1D5 and 6A12, which corresponded to the amino acid sequences (87)PPQGMAW(93) and (412)IQTRTEP(418), respectively, in the IBV S1 subunit. Sequences comparison revealed that epitope (412)IQTRTEP(418) was conserved among IBVs, while the epitope (87)PPQGMAW(93) was relatively variable among IBVs. The novel mAbs and the epitopes identified will be usefu...Continue Reading

References

Jan 1, 1987·The Journal of General Virology·M E BoursnellM M Binns
Jan 1, 1995·Archives of Virology·L WangE W Collisson
Apr 9, 2010·Viral Immunology·Dan YuShengwang Liu

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Citations

Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jing XiaYong Huang
Nov 13, 2017·Virus Genes·Giuliana Loreto SaraivaAbelardo Silva-Júnior
Nov 11, 2019·Veterinary Research·Giovanni FranzoMattia Cecchinato
Mar 10, 2021·Current Microbiology·Taruna AnandBhupendra N Tripathi

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Datasets Mentioned

BETA
AY842862
EU526388
GU384206

Methods Mentioned

BETA
ELISA
phage display
electrophoresis

Software Mentioned

MegAlign
Lasergene

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