Abstract
The spike (S) protein of the infectious bronchitis virus (IBV) plays a central role in the pathogenicity, the immune antibody production, serotype and the tissue tropism. In this study, we generate 11 monoclonal antibodies (mAbs) against S1 subunit of IBV Sczy3 strain, and two mAbs 1D5 and 6A12 were positive in indirect ELISA against both His-S1 protein and the purified whole viral antigen. MAb 6A12 and 1D5 could recognized by other 10 IBV strains (IBVs) from five different genotypes, except that 1D5 had a relatively low reaction with two of the 10 tested IBVs. End-point neutralizing assay performed in chicken embro kidney (CEK) cells revealed that the neutralization titer of 6A12 and 1D5 against Sczy3 reached 1:44.7 and 1:40.6, respectively. After screening a phage display peptide library and peptide scanning, we identified two linear B-cell epitopes that were recognized by the mAbs 1D5 and 6A12, which corresponded to the amino acid sequences (87)PPQGMAW(93) and (412)IQTRTEP(418), respectively, in the IBV S1 subunit. Sequences comparison revealed that epitope (412)IQTRTEP(418) was conserved among IBVs, while the epitope (87)PPQGMAW(93) was relatively variable among IBVs. The novel mAbs and the epitopes identified will be usefu...Continue Reading
References
Mar 1, 1992·The Journal of General Virology·A KantB A van der Zeijst
Jul 1, 1986·The Journal of General Virology·D CavanaghR W Peters
Jan 1, 1987·The Journal of General Virology·M E BoursnellM M Binns
Jan 1, 1993·Archives of Virology·R L Parr, E W Collissor
Jan 1, 1995·Archives of Virology·L WangE W Collisson
Jun 24, 2000·Veterinary Microbiology·J N SeahJ Kwang
Jul 30, 2003·Journal of Virology·Rosa CasaisPaul Britton
May 4, 2005·Archives of Virology·J Ignjatovic, S Sapats
Mar 14, 2007·Zoonoses and Public Health·J Q HuJ Y Zhou
Mar 1, 1997·Avian Pathology : Journal of the W.V.P.A·D CavanaghJ K Cook
Jan 22, 2009·Avian Pathology : Journal of the W.V.P.A·S Abd El RahmanC Winter
Sep 30, 2009·Vaccine·Yun-Feng WangMei Wang
Oct 24, 2009·The Journal of Microbiology·Junji XingXiangang Kong
Dec 22, 2009·Veterinary Microbiology·Linlin LiYongchang Cao
Apr 9, 2010·Viral Immunology·Dan YuShengwang Liu
Jun 18, 2010·Virus Genes·Nian-Li ZouYong Huang
Nov 6, 2012·Virus Research·Zongxi HanShengwang Liu
Jan 29, 2013·Virus Genes·Fangfang ZhaoYong Huang
Dec 18, 2013·Vaccine·Jie ZhangHui-Ying Fan
Citations
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jing XiaYong Huang
Sep 27, 2016·Advances in Bioinformatics·Faruku BandeAbdul Rahman Omar
Sep 26, 2016·Vaccine·Lei TanChan Ding
Dec 19, 2019·Viruses·Shuyun LiYong Huang
Nov 13, 2017·Virus Genes·Giuliana Loreto SaraivaAbelardo Silva-Júnior
Nov 11, 2019·Veterinary Research·Giovanni FranzoMattia Cecchinato
Jan 27, 2021·BMC Veterinary Research·Qi WuAijian Qin
Mar 10, 2021·Current Microbiology·Taruna AnandBhupendra N Tripathi