Two novel peptides with angiotensin I converting enzyme inhibitory and antioxidative activities from Scorpaena notata muscle protein hydrolysate

Biotechnology and Applied Biochemistry
Neyssene AissaouiMohamed Nejib Marzouki

Abstract

Fish protein hydrolysate was prepared from muscle of small red scorpionfish (Scorpaena notata) by treatment with a protease from the fungus Penicillium digitatum. Protein hydrolysate was found to strongly inhibit the angiotensin I converting enzyme and exhibited high antioxidative activity through 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. After ultrafiltration, peptides were isolated by a two-step procedure: size exclusion chromatography on a Toyopearl HW-40 followed by reversed-phase high-performance liquid chromatography with a high purification yield of 2.5 mg of peptide per gram of initial protein. Two major peptides were then identified by nanoscale liquid chromatography coupled to tandem mass spectrometry (nano-LC-MS/MS), corresponding to the following sequences: Leu-Val-Thr-Gly-Asp-Asp-Lys-Thr-Asn-Leu-Lys (1,204.665 Da) and Asp-Thr-Gly-Ser-Asp-Lys-Lys-Gln-Leu (992.511 Da). These peptides, mainly composed of hydrophilic amino acids, showed high antioxidative and angiotensin I converting enzyme inhibitory activities. These data suggest that the two novel peptides isolated from the muscle hydrolysate of small red scorpionfish can be a beneficial ingredient for functional foods or pharmaceuticals against h...Continue Reading

References

Dec 1, 1994·Bioscience, Biotechnology, and Biochemistry·H MatsufujiY Osajima
Nov 25, 2000·The Journal of Nutritional Biochemistry·K Suetsuna, T Nakano
May 29, 2003·Journal of Agricultural and Food Chemistry·Ai SaigaToshihide Nishimura
Sep 19, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·He Hai-LunZhou Bai-Cheng
Mar 16, 2013·Applied Biochemistry and Biotechnology·Ferid AbidiMohamed Nejib Marzouki

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Citations

Nov 23, 2017·Biotechnology and Applied Biochemistry·Jiraporn LueangsakulthaiSompong Klaynongsruang
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·José Antonio VázquezJesus Valcarcel
Feb 18, 2021·Journal of Agricultural and Food Chemistry·Yanbo HuangSong-Qing Hu

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