Two Novel PLS-Class Pentatricopeptide Repeat Proteins Are Involved in the Group II Intron Splicing of Mitochondrial Transcripts in the Moss Physcomitrella patens.

Plant & Cell Physiology
Mizuho IchinoseMamoru Sugita

Abstract

Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that function in posttranscriptional regulation as gene-specific regulators of RNA metabolism in plant organelles. Plant PPR proteins are divided into four classes: P, PLS, E and DYW. The E- and DYW-class proteins are mainly implicated in RNA editing, whereas most of the P-class proteins predominantly participate in RNA cleavage, splicing and stabilization. In contrast, the functions of PLS-class proteins still remain obscure. Here, we report the function of PLS-class PpPPR_31 and PpPPR_9 in Physcomitrella patens. The knockout (KO) mutants of PpPPR_31 and PpPPR_9 exhibited slower protonema growth compared to the wild type. The PpPPR_31 KO mutants showed a considerable reduction in the splicing of nad5 intron 3 and atp9 intron 1. The PpPPR_9 KO mutants displayed severely reduced splicing of cox1 intron 3. An RNA electrophoresis mobility shift assay showed that the recombinant PpPPR_31 protein bound to the 5' region of nad5 exon 4 and the bulged A region in domain VI of atp9 group II intron 1 while the recombinant PpPPR_9 bound to the translated region of ORF622 in cox1 intron 3. These results suggest that a certain set of PLS-class PPR proteins may influence the sp...Continue Reading

References

Sep 1, 1989·Trends in Genetics : TIG·M W Gray
Jan 1, 1995·Annual Review of Biochemistry·F Michel, J L Ferat
Oct 1, 1996·Plant Molecular Biology·S BinderA Brennicke
Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Feb 9, 2000·Trends in Biochemical Sciences·I D Small, N Peeters
Mar 16, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T NishiyamaM Hasebe
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Dec 1, 2004·Annual Review of Genetics·Alan M Lambowitz, Steven Zimmerly
Oct 17, 2006·The Plant Cell·Christian Schmitz-LinneweberAlice Barkan
Apr 5, 2008·Science·Navtej ToorAnna Marie Pyle
Nov 14, 2008·Trends in Plant Science·Christian Schmitz-Linneweber, Ian Small
Feb 19, 2010·The Plant Journal : for Cell and Molecular Biology·Eiji TasakiMamoru Sugita
Mar 3, 2010·Annual Review of Plant Biology·David B SternMaureen R Hanson
May 12, 2011·The New Phytologist·Sota Fujii, Ian Small
Nov 29, 2011·The Plant Journal : for Cell and Molecular Biology·Mizuho IchinoseMamoru Sugita
Jan 12, 2012·Molecular BioSystems·Aleksandra Filipovska, Oliver Rackham
May 7, 2013·RNA Biology·Mamoru SugitaChieko Sugita
Jan 28, 2014·Trends in Plant Science·Kamel Hammani, Philippe Giegé
Jan 30, 2014·Annual Review of Plant Biology·Alice Barkan, Ian Small
Jan 28, 2015·Biochimica Et Biophysica Acta·Christian Schmitz-LinneweberOren Ostersetzer-Biran
Jan 15, 2016·The Plant Journal : for Cell and Molecular Biology·Shifeng ChengIan Small
Jan 15, 2016·The Plant Journal : for Cell and Molecular Biology·Zhihui XiuBao-Cai Tan
Apr 28, 2016·The Plant Journal : for Cell and Molecular Biology·Seiya GotoMamoru Sugita
Dec 13, 2016·Plant Molecular Biology·Stefan WeißenbergerChris Carrie
Dec 28, 2016·Genes·Mizuho Ichinose, Mamoru Sugita
Mar 28, 2017·The Plant Journal : for Cell and Molecular Biology·Manjun CaiFazhan Qiu

❮ Previous
Next ❯

Citations

Jul 21, 2020·Plant & Cell Physiology·Volker Knoop

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.