Two novel variants of the v-src oncogene isolated from low and high metastatic RSV-transformed hamster cells

Virology
A TatosyanP Dezélée

Abstract

Four different transformed cell lines were isolated as a result of independent infection of primary hamster fibroblasts by Rous sarcoma virus (RSV SR-D stocks). These lines differ by the level of their spontaneous metastatic activity: HET-SR-1, HET-SR-8, and HET-SR-10 cell lines induced 70-200 metastatic nodules in the lung and/or lymph nodes of inoculated animals (high metastatic lines, HM). Metastatic activity was not identified after injection of HET-SR cells (low metastatic line, LM). All cell lines contained one copy of integrated and expressed intact RSV provirus. The difference in the amount of v-src protein in cell lines was not correlated with their metastatic potential in vivo. Complete v-srcHM and v-srcLM genes were cloned from corresponding gene libraries and sequenced. In the unique region of both v-src isoforms a GC-rich insert of 60 nucleotides (20 a.a.) was found. The presence of this insert explains the unusual apparent molecular weight of protein encoded by v-srcHM and vsrcLM: 62 kDA. Both genes had 10 identical amino acid changes when compared to the known RSV SR-D v-src sequence. v-srcHM and v-srcLM differ by several amino acid changes. Most of them are localized in the unique domain and the extreme carboxy-...Continue Reading

Citations

Sep 20, 2011·Journal of Cellular Biochemistry·Anna V KnizhnikElena M Tchevkina
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Elina ZuevaBertrand Tavitian
Oct 9, 1996·International Journal of Cancer. Journal International Du Cancer·R L TaylorM S Halpern
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette JeulinFrancelyne Marano
Nov 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Haiying ChengGeoffrey W G Sharp
Jun 28, 2001·The Journal of Biological Chemistry·O MizeninaA Tatosyan

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