Two nuclear effectors of the rice blast fungus modulate host immunity via transcriptional reprogramming

Nature Communications
Seongbeom KimYong-Hwan Lee

Abstract

Pathogens utilize multiple types of effectors to modulate plant immunity. Although many apoplastic and cytoplasmic effectors have been reported, nuclear effectors have not been well characterized in fungal pathogens. Here, we characterize two nuclear effectors of the rice blast pathogen Magnaporthe oryzae. Both nuclear effectors are secreted via the biotrophic interfacial complex, translocated into the nuclei of initially penetrated and surrounding cells, and reprogram the expression of immunity-associated genes by binding on effector binding elements in rice. Their expression in transgenic rice causes ambivalent immunity: increased susceptibility to M. oryzae and Xanthomonas oryzae pv. oryzae, hemibiotrophic pathogens, but enhanced resistance to Cochliobolus miyabeanus, a necrotrophic pathogen. Our findings help remedy a significant knowledge deficiency in the mechanism of M. oryzae-rice interactions and underscore how effector-mediated manipulation of plant immunity by one pathogen may also affect the disease severity by other pathogens.

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Citations

Apr 22, 2021·Fungal Genetics and Biology : FG & B·Alice Bisola EseolaNicholas J Talbot
May 17, 2021·Current Opinion in Plant Biology·Melania FigueroaEva C Henningsen
Aug 13, 2021·Journal of Integrative Plant Biology·Muhammad TariqjaveedWenxian Sun
Aug 21, 2021·Molecular Plant-microbe Interactions : MPMI·Kyungyong Seong, Ksenia V Krasileva

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Methods Mentioned

BETA
transgenic
RNA-seq
chip
one-hybrid
light microscopy
Assay
phosphotransferase
PCR

Software Mentioned

BLAST Genomics Platform
NLStradamus
WoLF
BLASTMatrix
CFGP2
cNLS mapper
NGS QC Toolkit
HISAT2
StringTie
Axiovision

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