Two-omics data revealed commonalities and differences between Rpv12- and Rpv3-mediated resistance in grapevine.

Scientific Reports
Giulia ChitarriniUrska Vrhovsek

Abstract

Plasmopara viticola is the causal agent of grapevine downy mildew (DM). DM resistant varieties deploy effector-triggered immunity (ETI) to inhibit pathogen growth, which is activated by major resistance loci, the most common of which are Rpv3 and Rpv12. We previously showed that a quick metabolome response lies behind the ETI conferred by Rpv3 TIR-NB-LRR genes. Here we used a grape variety operating Rpv12-mediated ETI, which is conferred by an independent locus containing CC-NB-LRR genes, to investigate the defence response using GC/MS, UPLC, UHPLC and RNA-Seq analyses. Eighty-eight metabolites showed significantly different concentration and 432 genes showed differential expression between inoculated resistant leaves and controls. Most metabolite changes in sugars, fatty acids and phenols were similar in timing and direction to those observed in Rpv3-mediated ETI but some of them were stronger or more persistent. Activators, elicitors and signal transducers for the formation of reactive oxygen species were early observed in samples undergoing Rpv12-mediated ETI and were paralleled and followed by the upregulation of genes belonging to ontology categories associated with salicylic acid signalling, signal transduction, WRKY tran...Continue Reading

References

Oct 10, 1998·Plant Physiology·D M RibnickyI Raskin
Feb 8, 2002·Trends in Plant Science·Martin Heil, Ian T Baldwin
Jul 25, 2003·Trends in Plant Science·Kirk OvermyerJaakko Kangasjärvi
Oct 24, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B M FischerE M Zyprian
Dec 9, 2003·Journal of Plant Physiology·Andreas Kortekamp, Eva Zyprian
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Alisdair R FernieLothar Willmitzer
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Takashi YaenoKoh Iba
Jun 10, 2006·BMC Genomics·Robert A van den BergMariët J van der Werf
Nov 24, 2006·PLoS Pathogens·Thomas Eulgem
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Colleen KnothThomas Eulgem
Aug 28, 2007·Nature·Olivier JaillonUNKNOWN French-Italian Public Consortium for Grapevine Genome Characterization
Apr 8, 2009·Molecular Plant-microbe Interactions : MPMI·Melvin D Bolton
May 1, 2006·Plant Signaling & Behavior·Benoît RantyJean-Philippe Galaud
Oct 13, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Diana BellinGabriele Di Gaspero
Dec 15, 2010·Current Protocols in Bioinformatics·Ben Langmead
Jul 8, 2011·Planta·Karen CasagrandeGabriele Di Gaspero
Sep 22, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Florian SchwanderReinhard Töpfer
Feb 4, 2012·The Arabidopsis Book·D'Maris Amick DempseyDaniel F Klessig
Apr 4, 2012·Journal of Agricultural and Food Chemistry·Urska VrhovsekFulvio Mattivi
May 4, 2012·Journal of Experimental Botany·Mohammad Reza Bolouri Moghaddam, Wim Van den Ende
Jun 12, 2012·Plant Science : an International Journal of Experimental Plant Biology·Kashif AliYoung Hae Choi
Feb 28, 2014·Frontiers in Plant Science·Clemencia M RojasKirankumar S Mysore
Jun 3, 2014·BMC Bioinformatics·Chen MengAmin Moghaddas Gholami
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 14, 2015·Journal of Experimental Botany·Patricia Agudelo-RomeroAna Margarida Fortes
Jun 5, 2015·Microbiology and Molecular Biology Reviews : MMBR·Stuart FawkeSebastian Schornack

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Methods Mentioned

BETA
PCA
PCR
transgenic
RNA-Seq

Software Mentioned

R
TopHat2
Co
MCIA
Regent
R package omicade4
Waters MassLynx
GATK
Bowtie2
LC480

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