Two overlapping domains of a lyssavirus matrix protein that acts on different cell death pathways.

Journal of Virology
Florence LarrousHervé Bourhy

Abstract

The lyssavirus matrix (M) protein induces apoptosis. The regions of the M protein that are essential for triggering cell death pathways are not yet clearly defined. We therefore compared the M proteins from two viruses that have contrasting characteristics in terms of cellular apoptosis: a genotype 3 lyssavirus, Mokola virus (MOK), and a genotype 1 rabies virus isolated from a dog from Thailand (THA). We identified a 20-amino-acid fragment (corresponding to positions 67 to 86) that retained the cell death activities of the full-length M protein from MOK via both the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and inhibition of cytochrome c oxidase (CcO) activity. We found that the amino acids at positions 77 and 81 have an essential role in triggering these two cell death pathways. Directed mutagenesis demonstrated that the amino acid at position 77 affects CcO activity, whereas the amino acid at position 81 affects TRAIL-dependent apoptosis. Mutations in the full-length M protein that compromised induction of either of these two pathways resulted in delayed apoptosis compared with the time to apoptosis for the nonmutated control.

References

Jun 1, 1994·Journal of Virology·V S HinshawD Evans
Oct 6, 1997·Journal of Virology·M I ThoulouzeM Lafon
Apr 28, 1999·Trends in Microbiology·H Everett, G McFadden
Aug 10, 2000·Journal of Virology·P ClarkeK L Tyler
May 31, 2001·Journal of Cellular Physiology·B GuanS Y Sun
Oct 31, 2002·Nature Immunology·Chris A BenedictCarl F Ware
Oct 31, 2003·Biochimie·Leïla Baloul, Monique Lafon
Mar 23, 2004·Annals of the New York Academy of Sciences·Maria-Isabel ThoulouzeMonique Lafon
Sep 11, 2004·Biochemical and Biophysical Research Communications·Mi-Hyang KimDai-Wu Seol
Jun 13, 2006·Scandinavian Journal of Immunology·V V Sumbayev, I M Yasinska
Aug 23, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Satoshi Uematsu, Shizuo Akira
Dec 28, 2006·Journal of Biochemistry·Taro Kawai, Shizuo Akira
Aug 19, 2007·Apoptosis : an International Journal on Programmed Cell Death·Xiaohong LiangWensheng Sun
Mar 7, 2008·Journal of Virology·Alireza GholamiHervé Bourhy
Apr 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·René AssenbergJonathan M Grimes
May 1, 2008·PloS One·Olivier DelmasHervé Bourhy
Jun 3, 2008·PLoS Pathogens·Lorenzo GalluzziGuido Kroemer
Jun 5, 2008·The Journal of Clinical Investigation·Avi Ashkenazi, Roy S Herbst
Jan 16, 2009·Epidemiology and Infection·W MarkotterL H Nel
Jan 21, 2010·Science Signaling·Christophe PréhaudMonique Lafon

❮ Previous
Next ❯

Citations

Apr 23, 2013·Lancet Neurology·Thiravat HemachudhaJiraporn Laothamatas
Dec 15, 2010·PLoS Neglected Tropical Diseases·Hervé BourhyJérôme Salomon
Jan 29, 2013·Médecine sciences : M/S·Florence Ribadeau-DumasHervé Bourhy
Jun 13, 2015·Virus Research·Alexander Ghanem, Karl-Klaus Conzelmann
Jul 19, 2016·Experimental Cell Research·Jie ZanJi-Yong Zhou
Aug 23, 2016·Viruses·Terence P Scott, Louis H Nel
Oct 11, 2012·Cellular Microbiology·Reiko PollinStefan Finke
Nov 23, 2016·Frontiers in Microbiology·Sadegh Azimzadeh JamalkandiMohieddin Jafari

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis