Two Phages, phiIPLA-RODI and phiIPLA-C1C, Lyse Mono- and Dual-Species Staphylococcal Biofilms

Applied and Environmental Microbiology
Diana GutiérrezPilar García

Abstract

Phage therapy is a promising option for fighting against staphylococcal infections. Two lytic phages, vB_SauM_phiIPLA-RODI (phiIPLA-RODI) and vB_SepM_phiIPLA-C1C (phiIPLA-C1C), belonging to the Myoviridae family and exhibiting wide host ranges, were characterized in this study. The complete genome sequences comprised 142,348 bp and 140,961 bp and contained 213 and 203 open reading frames, respectively. The gene organization was typical of Spounavirinae members, with long direct terminal repeats (LTRs), genes grouped into modules not clearly separated from each other, and several group I introns. In addition, four genes encoding tRNAs were identified in phiIPLA-RODI. Comparative DNA sequence analysis showed high similarities with two phages, GH15 and 676Z, belonging to the Twort-like virus genus (nucleotide identities of >84%); for phiIPLA-C1C, a high similarity with phage phiIBB-SEP1 was observed (identity of 80%). Challenge assays of phages phiIPLA-RODI and phiIPLA-C1C against planktonic staphylococcal cells confirmed their lytic ability, as they were able to remove 5 log units in 8 h. Exposure of biofilms to phages phiIPLA-RODI and phiIPLA-C1C reduced the amount of adhered bacteria to about 2 log units in both monospecies and...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1988·Computer Applications in the Biosciences : CABIOS·E W Myers, W Miller
Aug 5, 2000·Journal of Molecular Biology·M D ErmolaevaS L Salzberg
Apr 9, 2001·Journal of Bacteriology·C CucarellaJ R Penadés
May 20, 2003·Molecular Microbiology·Jaione ValleIñigo Lasa
Jan 6, 2004·Nucleic Acids Research·Dean Laslett, Bjorn Canback
Apr 6, 2004·FEMS Microbiology Letters·Ian W SutherlandKaren Tait
Jun 28, 2005·Nucleic Acids Research·Peter SchattnerTodd M Lowe
Sep 10, 2005·Applied and Environmental Microbiology·W R Harcombe, J J Bull
Apr 16, 2008·Nucleic Acids Research·Jason R Grant, Paul Stothard
Sep 17, 2008·Infection and Immunity·M Shamim Hasan ZahidShah M Faruque
Jan 13, 2009·Infectious Disease Clinics of North America·Kathie L RogersMark E Rupp
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jun 13, 2009·Environmental Microbiology·Andrew M KropinskiRob Lavigne
Jul 18, 2009·Nature Reviews. Microbiology·Michael Otto
Oct 20, 2009·Applied and Environmental Microbiology·Pilar GarcíaAna Rodríguez
Mar 30, 2010·Nature Reviews. Microbiology·Simon J LabrieSylvain Moineau
May 8, 2010·Current Microbiology·Diana GutiérrezPilar García
Jun 10, 2010·FEMS Immunology and Medical Microbiology·Louise CarsonBrendan F Gilmore
Aug 18, 2010·Archives of Virology·Jochen KlumppHans-Wolfgang Ackermann
Sep 3, 2010·Trends in Biotechnology·Mzia Kutateladze, Revaz Adamia
Oct 30, 2010·Virology Journal·David R EdgellMarlene Belfort
Dec 15, 2010·Applied and Environmental Microbiology·Sue-Er HsiehYi-Hsiung Tseng
Feb 2, 2011·RNA Biology·Magali NavilleDaniel Gautheret
Jun 7, 2011·Journal of Bacteriology·Guoqing XiaAndreas Peschel
Sep 6, 2011·Applied and Environmental Microbiology·Ana B CampeloBeatriz Martínez
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Saravanan PeriasamyMichael Otto
Jan 24, 2012·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Virginia MartínEsther Jiménez
Jul 4, 2012·Advances in Virus Research·Ryszard MiędzybrodzkiAndrzej Górski
Jul 4, 2012·Advances in Virus Research·Małgorzata ŁobockaAleksandra Głowacka
Oct 2, 2012·Applied and Environmental Microbiology·Diana GutiérrezPilar García

❮ Previous
Next ❯

Citations

Jul 5, 2016·Frontiers in Microbiology·Diana GutiérrezPilar García
Aug 30, 2016·Frontiers in Microbiology·Beata Weber-DąbrowskaAndrzej Górski
Sep 20, 2016·Journal of Oral Microbiology·Leron KhalifaRonen Hazan
Nov 9, 2017·Antibiotics·Lucía FernándezAna Rodríguez
Jan 30, 2019·Journal of Cellular Physiology·Javad Yasbolaghi SharahiAli Hashemi
Mar 28, 2020·Antibiotics·Mikeljon P Nikolich, Andrey A Filippov
Jun 15, 2019·Archives of Virology·Salwa GomaaHisham Abbas
Mar 14, 2020·Journal of Global Infectious Diseases·Sonia Bhonchal BhardwajJyoti Sharma
Sep 16, 2018·Viruses·Eva González-MenéndezPilar García
Sep 7, 2018·Frontiers in Cellular and Infection Microbiology·Lucía FernándezPilar García
Feb 7, 2019·Frontiers in Microbiology·Diana GutiérrezPilar García
May 31, 2019·Biofouling·Legesse Geredew KifelewPeter Speck
Aug 18, 2018·Scientific Reports·Leonardo Joaquim van ZylMarla Trindade
Jun 30, 2019·Microbiology Resource Announcements·Miranda E FreemanMei Liu
Aug 25, 2019·Antibiotics·Lucía FernándezAna Rodríguez
Feb 11, 2020·Frontiers in Microbiology·Diana GutiérrezPilar García
Sep 8, 2019·Applied and Environmental Microbiology·Kirill V SergueevMikeljon P Nikolich
May 31, 2020·FEMS Microbiology Reviews·Diana P PiresJoana Azeredo
Dec 31, 2020·Antibiotics·Cristina UruénJesús Arenas
Feb 13, 2021·Antibiotics·Christian KranjecDzung B Diep
Jul 3, 2017·Journal of Theoretical Biology·Chung Yin Joey Leung, Joshua S Weitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.