Two-photon confocal microscopy: a nondestructive method for studying wound healing

Plastic and Reconstructive Surgery
Fernando A NavarroDennis P Orgill

Abstract

Two-photon confocal microscopy is a new technology useful in nondestructive analysis of tissue. The pattern generated from laser-excited autofluorescence and second harmonic signals can be analyzed to construct a three-dimensional, microanatomical, structural image. The healing of full-thickness guinea pig skin wounds was studied over a period of 28 days using two-photon confocal microscopy. Three-dimensional data were rendered from two-dimensional images and compared with conventional, en face, histologic sections. Two-photon confocal microscopy images show resolution of muscle, fascia fibers, collagen fibers, inflammatory cells, blood vessels, and hair. Although these images do not currently have the resolution of standard histology, the ability to noninvasively acquire three-dimensional images of skin promises to be an important tool in wound-healing studies.

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Citations

Oct 21, 2010·Lasers in Medical Science·Xiaoqin ZhuBifang Lin
Oct 11, 2008·Tissue Engineering. Part B, Reviews·Irene GeorgakoudiDavid L Kaplan
Jan 24, 2009·Photochemistry and Photobiology·Kerry M Hanson, Christopher J Bardeen
Sep 29, 2009·Seminars in Cutaneous Medicine and Surgery·John PaoliMarica B Ericson
Jun 10, 2016·Journal of Cell Science·Tom ShearerSarah H Cartmell
Jan 23, 2008·Archives of Facial Plastic Surgery·Victor Da CostaBrian J-F Wong

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