PMID: 11908849Mar 23, 2002Paper

Two-photon excitation and photolysis by pulsed laser illumination modelled by spatially non-uniform reactions with simultaneous diffusion

European Biophysics Journal : EBJ
Nikolai I Kiskin, David Ogden

Abstract

Two-photon absorption in the focus of a pulsed laser has the potential for localized photolysis of caged compounds, generating high concentrations of neurotransmitters, hormones and messengers. The concentrations of cage, intermediates and products in the femtolitre focal volume depend on reaction rates and diffusional exchange with the external volume. This problem of reaction with diffusion was analysed with analytical and numerical methods to determine simple relations between parameters useful in the design and interpretation of experiments. The diffraction-limited laser spot is approximated well by a sphere, radius A, in diffusional exchange with either an infinite uniform medium, representing extracellular photolysis, or within a non-permeable sphere, a "cell" of radius B, representing intracellular photolysis. Photolysis is modelled as sequential irreversible reactions, with either the excitation step alone, rate constant k(e), or with a subsequent "dark" reaction, rate constant k(p). For extracellular photolysis, steady-state depletion of a cage averaged in a spherical spot increases hyperbolically with k(e) with half-maximum depletion at k(e) = K0.5 = 2.5 D/A2, where D is the diffusion coefficient. With measured parame...Continue Reading

Citations

Feb 27, 2007·Pflügers Archiv : European journal of physiology·Eric A SobieW J Lederer
Aug 20, 2010·Journal of Cell Science·Nikolai I KiskinTom Carter
Jan 12, 2010·Journal of Biomedical Optics·Bradley E LosavioPeter Saggau
Dec 5, 2014·The European Journal of Neuroscience·Joseph M AmatrudoGraham C R Ellis-Davies
Dec 18, 2013·Chemistry & Biology·Adam C ReaTimothy M Dore
Sep 26, 2003·Biophysical Journal·Nikolai I KiskinReiner Peters
Feb 24, 2018·Chembiochem : a European Journal of Chemical Biology·Alexander P GorkaMartin J Schnermann
Feb 3, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jérôme QuérardLudovic Jullien
Feb 19, 2013·Angewandte Chemie·Guillaume BortPeter I Dalko
Jun 18, 2014·Nature Chemical Biology·Arnaud GautierSophie Vriz
Oct 20, 2017·ChemistryOpen·Petra DunkelPeter I Dalko
Jan 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Norman P ScheppFrances L Cozens

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