Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber.

Optics Express
Yu-Chung ChangT Norris

Abstract

We report on the utilization of a dual-clad optical fiber for two-photon excited fluorescence correlation spectroscopy (FCS). High excitation efficiency is obtained by sending prechirped ultrafast pulses through the single-mode core of the fiber, while the fluorescence collection efficiency is enhanced because of the larger numerical aperture of the inner cladding. We show that the utilization of a dual-clad fiber is critical for ultrasensitive two-photon fluorescence detection. Our system has the ability to detect fluorescent nanospheres as small as 12 nm in radius. Quantum dots of radius 7 nm are also measured and show excellent signal to noise ratio. The particle sizes obtained from the fiber FCS system were confirmed by measurements using a commercial dynamic light scattering (DLS) system.

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Citations

Dec 18, 2015·Scientific Reports·Guillaume DucourthialFrédéric Louradour
Jul 29, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Eric R Tkaczyk, Alan H Tkaczyk
Apr 8, 2010·Optics Express·Heykel AouaniHervé Rigneault
Jan 25, 2011·Biomedical Optics Express·Youbo ZhaoRobert J Gordon
Jan 1, 2017·Light, Science & Applications·Wenxuan LiangXingde Li
Aug 31, 2010·Journal of Biomedical Optics·Yu-Chung ChangTheodore B Norris
Nov 16, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Vytautas KučikasMarc A M J van Zandvoort

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