Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Uta GehlsenPhilipp Steven

Abstract

Early and correct diagnosis of delayed or absent corneal epithelial wound healing is a key factor in the prevention of infection and consecutive destruction of the corneal stroma with impending irreversible visual loss. Two-photon microscopy (TPM) is a novel technology that has potential to depict epithelial cells and to evaluate cellular function by measuring autofluorescence properties such as fluorescence intensity and fluorescence lifetimes of metabolic co-factors such as NAD(P)H. Using non-invasive TPM in a tissue-culture scratch model and an organ-culture erosion model, fluorescence intensity and fluorescence lifetimes of NAD(P)H were measured before and during closure of the epithelial wounds. Influence of temperature and selective inhibition of metabolism on intensity and lifetimes were tested additionally. Decrease of temperature resulted in significant increase of fluorescence lifetimes and decrease of the relative amount of free NAD(P)H due to decreased global metabolism. Increase in temperature and upregulation of glycolysis through blocking the mitochondrial electron transport chain by rotenone resulted in increased intensity, decreased lifetimes and increase in the relative amount of free NAD(P)H. Changes of lifet...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R LakowiczM L Johnson
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Dec 9, 2004·Immunology Letters·Eleanor P W Kable, Alexandra K Kiemer
Dec 25, 2004·Journal of Fluorescence·Herbert SchneckenburgerReinhard Sailer
May 3, 2005·The Journal of Biological Chemistry·Harshad D VishwasraoWatt W Webb
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melissa C SkalaNirmala Ramanujam
Mar 11, 2008·The Journal of Biological Chemistry·Friedrich P PaulsenSaadettin Sel
Apr 4, 2008·Investigative Ophthalmology & Visual Science·Philipp StevenAndreas Gebert
Sep 17, 2008·Optics Letters·Andrew J RadosevichElizabeth M C Hillman
Jan 17, 2009·Optics Letters·Dong LiJianan Y Qu
Aug 14, 2009·American Journal of Ophthalmology·Jay C ErieSanjay V Patel
Jan 12, 2010·Journal of Biomedical Optics·Philipp StevenGereon Hüttmann
Jan 29, 2010·Klinische Monatsblätter für Augenheilkunde·G HüttmannR Birngruber
May 27, 2010·Developments in Ophthalmology·U GehlsenP Steven

❮ Previous
Next ❯

Citations

May 31, 2014·Nature Communications·Thomas S BlackerMichael R Duchen
Aug 16, 2016·Free Radical Biology & Medicine·Thomas S Blacker, Michael R Duchen
Aug 29, 2019·Biomedical Engineering Letters·Harsh RanawatNirmal Mazumder
Jun 10, 2018·Scientific Reports·Michael EversDieter Manstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Jorge VergenMichael G Nichols
Proceedings of the National Academy of Sciences of the United States of America
Melissa C SkalaNirmala Ramanujam
Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
D SchweitzerJ Dawczynski
© 2021 Meta ULC. All rights reserved