Two-photon fluorescent probe for detection of nitroreductase and hypoxia-specific microenvironment of cancer stem cell

Analytica Chimica Acta
Yajing LiuJinchao Zhang

Abstract

Hypoxia plays a crucial role in cancer progression, and it has great significance for monitoring hypoxic level in biosystems. Cancer stem cells (CSCs) represent a small population of tumour cells that regard as the key to seed tumours. The survival of CSCs depend on the tumour microenvironment, which is distinct region has the hypoxic property. Therefore, the detection of the hypoxic CSC niche plays a pivotal role in the destructing the 'soil' of CSCs, and eliminating CSCs population. Numerous one-photon excited fluorescent probes have been developed to indicate the hypoxic status in tumours through the detection of nitroreductase (NTR) level. However, the biomedical application of one-photon fluorescent probes is limited due to the poor tissue penetration. In the present work, we reported a two-photon fluorescent probe to detect the NTR in CSCs and monitor the hypoxic microenvironment in vivo. The two-photon fluorescent molecular probe with a hypoxic specific response group can be reduced by NTR under hypoxic conditions. We used the two-photon probe to detect the hypoxia status of 3D cultured-CSCs in vitro and in vivo CSCs' microenvironment in tumour. The two-photon absorption cross section extends fluorescent excitation spect...Continue Reading

Citations

May 7, 2020·Antioxidants & Redox Signaling·Gabriel Gojon, Guillermo A Morales
Oct 12, 2018·International Journal of Molecular Sciences·Stéphane TerrySalem Chouaib
Jul 2, 2020·Organic & Biomolecular Chemistry·Jie GaoXianfeng Gu
Nov 27, 2020·Advanced Healthcare Materials·Miffy Hok Yan ChengGang Zheng
Apr 9, 2020·Sensors and Actuators. B, Chemical·Jessica L KlockowFrederick T Chin
Apr 4, 2021·Cancer Reports·Kenneth S HettieFrederick T Chin
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Feng LiuZhihui Li

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