Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks

Nature Communications
Teppei EbinaMasanori Matsuzaki

Abstract

Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2-5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show direction-selective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.

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Citations

Mar 5, 2020·Journal of Neurophysiology·Jeffrey D WalkerNicholas G Hatsopoulos
Sep 5, 2018·Nature Communications·Shin-Ichiro TeradaMasanori Matsuzaki
Oct 20, 2018·Biomedical Optics Express·Gregorio L GaliñanesDaniel Huber
Oct 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Teppei EbinaMasanori Matsuzaki
Jul 28, 2020·NeuroImage·Robert M FriedmanOmar A Gharbawie
Jun 19, 2021·Nature Communications·Eric M TrautmannKrishna V Shenoy
Jul 20, 2021·Current Opinion in Pharmacology·Tetsuo Yamamori
Jul 9, 2021·Annual Review of Neuroscience·Hideyuki Okano
Jul 13, 2021·Biochemistry·Xinqi ZhouEvan W Miller
Jul 21, 2021·Microscopy·Alice M StamatakisKunal K Ghosh
Aug 4, 2021·Nature Communications·Masanori Matsuzaki, Teppei Ebina

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Methods Mentioned

BETA
imaging technique
transgenic

Software Mentioned

ImageJ
Visual C + + 2008
SW
NoRMCorre
FV30S
MathWorks
LabView
R
CNMF
MATLAB

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