Two-photon laser scanning microscopy with electrowetting-based prism scanning

Biomedical Optics Express
Omkar D SupekarVictor M Bright

Abstract

Laser scanners are an integral part of high resolution biomedical imaging systems such as confocal or 2-photon excitation (2PE) microscopes. In this work, we demonstrate the utility of electrowetting on dielectric (EWOD) prisms as a lateral laser-scanning element integrated in a conventional 2PE microscope. To the best of our knowledge, this is the first such demonstration for EWOD prisms. EWOD devices provide a transmissive, low power consuming, and compact alternative to conventional adaptive optics, and hence this technology has tremendous potential. We demonstrate 2PE microscope imaging of cultured mouse hippocampal neurons with a FOV of 130 × 130 μm2 using EWOD prism scanning. In addition, we show simulations of the optical system with the EWOD prism, to evaluate the effect of propagating a Gaussian beam through the EWOD prism on the imaging quality. Based on the simulation results a beam size of 0.91 mm full width half max was chosen to conduct the imaging experiments, resulting in a numerical aperture of 0.17 of the imaging system.

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Citations

Mar 27, 2019·Scientific Reports·Chao LiuQiong-Hua Wang
Jul 31, 2019·Biomedical Optics Express·Kayvan Forouhesh TehraniLuke J Mortensen
Mar 17, 2019·Optics Express·Mo ZohrabiJuliet T Gopinath
Nov 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Wei Yang LimVictor M Bright

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