Two-photon microscopy of Paneth cells in the small intestine of live mice

Scientific Reports
Won Hyuk JangKi Hean Kim

Abstract

Paneth cells are one of the principal epithelial cell types in the small intestine, located at the base of intestinal crypts. Paneth cells play key roles in intestinal host-microbe homeostasis via granule secretion, and their dysfunction is implicated in pathogenesis of several diseases including Crohn's disease. Despite their physiological importance, study of Paneth cells has been hampered by the limited accessibility and lack of labeling methods. In this study, we developed a simple in vivo imaging method of Paneth cells in the intact mouse small intestine by using moxifloxacin and two-photon microscopy (TPM). Moxifloxacin, an FDA-approved antibiotic, was used for labeling cells and its fluorescence was strongly observed in Paneth cell granules by TPM. Moxifloxacin labeling of Paneth cell granules was confirmed by molecular counterstaining. Comparison of Paneth cells in wild type, genetically obese (ob/ob), and germ-free (GF) mice showed different granule distribution. Furthermore, Paneth cell degranulation was observed in vivo. Our study suggests that TPM with moxifloxacin labeling can serve as a useful tool for studying Paneth cell biology and related diseases.

References

Apr 6, 1990·Science·W DenkW W Webb
Jun 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D S ReillyM Zasloff
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Warren R ZipfelWatt W Webb
Jul 10, 2003·Gastroenterology·Sanjay LalaSatish Keshav
Jan 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lane L ClarkeNancy M Walker
Jan 24, 2004·Clinical and Experimental Immunology·P KellyW Dhaliwal
Apr 2, 2004·The Journal of Antimicrobial Chemotherapy·Martin WirtzHelmut Friess
Jul 28, 2004·The American Journal of Pathology·Cristiano RumioAndrea Balsari
Feb 15, 2005·Journal of Pharmaceutical and Biomedical Analysis·Marie-Hélène LangloisMarie-Claude Saux
Nov 1, 2005·Survey of Ophthalmology·Stella M RobertsonDavid C Dahlin
Nov 1, 2005·Survey of Ophthalmology·David H McGeeRebecca L Rice
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jan WehkampCharles L Bevins
Aug 10, 2006·The Journal of Antimicrobial Chemotherapy·H StassK-H Vestweber
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan WehkampEduard F Stange
Jun 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Donghai WangLeonard H Augenlicht
Nov 25, 2011·Journal of Biomedical Optics·Regina Orzekowsky-SchroederGereon Hüttmann
Feb 13, 2013·Annual Review of Physiology·Hans C Clevers, Charles L Bevins
Feb 23, 2013·Nature Protocols·Laila RitsmaJacco van Rheenen
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
Nov 19, 2013·Seminars in Immunology·Nita H Salzman, Charles L Bevins
Feb 26, 2014·Nature Reviews. Immunology·Lance W Peterson, David Artis
Jun 3, 2014·BMC Gastroenterology·Naomi SimmondsAlan W H Bates
Jul 2, 2014·The Journal of Experimental Medicine·Henner F FarinHans Clevers
Mar 25, 2015·Journal of Immunological Methods·Masha KolesnikovGuy Shakhar
Jun 19, 2015·Clinical and Translational Gastroenterology·Parth J ParekhDavid A Johnson
Aug 4, 2015·Nature Immunology·Qin ZhangZhihua Liu
Oct 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lioba F CourthJan Wehkamp
Jun 11, 2016·Scientific Reports·Taejun WangKi Hean Kim
Oct 13, 2016·Gastroenterology·Thaddeus S Stappenbeck, Dermot P B McGovern

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Citations

May 18, 2019·Communications Biology·Andrew ChojnackiKamala D Patel
Nov 6, 2020·Biomedical Optics Express·Seunghun LeeKi Hean Kim
Nov 28, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vladimir N NikolenkoMikhail Y Sinelnikov
Jan 12, 2020·Communications Biology·Andrew ChojnackiKamala D Patel

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MATLAB
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MathWorks
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