Two-Photon Microscopy with a Double-Wavelength Metasurface Objective Lens

Nano Letters
Ehsan ArbabiAndrei Faraon

Abstract

Two-photon microscopy is a key imaging technique in life sciences due to its superior deep-tissue imaging capabilities. Light-weight and compact two-photon microscopes are of great interest because of their applications for in vivo deep brain imaging. Recently, dielectric metasurfaces have enabled a new category of small and lightweight optical elements, including objective lenses. Here we experimentally demonstrate two-photon microscopy using a double-wavelength metasurface lens. It is specifically designed to focus 820 and 605 nm light, corresponding to the excitation and emission wavelengths of the measured fluorophors, to the same focal distance. The captured two-photon images are qualitatively comparable to the ones taken by a conventional objective lens. Our metasurface lens can enable ultracompact two-photon microscopes with similar performance compared to current systems that are usually based on graded-index-lenses. In addition, further development of tunable metasurface lenses will enable fast axial scanning for volumetric imaging.

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Citations

May 11, 2019·Micromachines·Bo LiZhen Qiu
Apr 29, 2020·Biomedical Optics Express·Antu Nehuen GortariAlejandro Yacomotti
Sep 16, 2020·Optics Letters·Lili TangZheng-Gao Dong
Dec 12, 2019·Advanced Materials·Wenbo ZangShining Zhu
Dec 22, 2020·IScience·Seong-Won MoonJunsuk Rho
Jun 8, 2021·Nano Letters·Yuan LuoDin Ping Tsai
Aug 25, 2021·Nano Letters·Yubin FanShumin Xiao
Sep 23, 2021·Nature Communications·Yujie WangShumin Xiao
Oct 1, 2019··Wei Ting ChenFederico Capasso
Feb 27, 2019··Seyedeh Mahsa KamaliAndrei Faraon

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