Two plant-hosted whole-cell bacterial biosensors for detection of bioavailable Cr(VI)

World Journal of Microbiology & Biotechnology
Romeu FranciscoPaula V Morais

Abstract

Metal whole-cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 μM), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, togeth...Continue Reading

References

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Dec 20, 2017·World Journal of Microbiology & Biotechnology·Romeu FranciscoPaula V Morais

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Citations

Mar 13, 2021·Frontiers in Bioengineering and Biotechnology·Ni ZengPeng Cai

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Methods Mentioned

BETA
biosensors
biosensing
PCR
biosensor
atomic absorption spectroscopy
fluorescence microscopy
Fluorescence
confocal microscopy

Software Mentioned

Leica Application Suite Lite
GraphPad
Fiji ImageJA
GraphPad Prism
ImageJ
ImageQuant TL

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