PMID: 9541092Apr 16, 1998Paper

Two populations of endothelial cell antibodies cross-react with heparin

Lupus
Y RenaudineauR Le Corre

Abstract

As endothelial cells (EC) express heparin-like glycosaminoglycans, such as heparan sulfate, it was essential to investigate the relation of anti-EC antibody (AECA) to heparin reactivity. AECA were detected in 43 of 131 autoimmune sera and anti-heparin antibodies (AHA) in 25. These autoimmune reactivities were significantly associated (P corrected < 0.0005). Seven AECA-positive/AHA-positive and three AECA-negative/AHA-positive sera were affinity-purified using protein G column followed by a heparin-Sepharose column. Two populations of AECA were recovered from the second column. One was eluted with 0.4 M NaCl which bound to EC and to solid-phase heparin with low affinity, but not to soluble heparin. The second population of AECA, which was eluted with 4 M guanidine HCl/2 M NaCl, recognized EC and solid-phase heparin with high affinity, but also soluble heparin. The latter population of AECA might thus be an important cause of autoimmune vascular thrombosis in systemic diseases.

References

Nov 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·M B Zucker, I R Katz
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Jul 1, 1990·The Journal of Clinical Investigation·P M Rumore, C R Steinman
Mar 5, 1987·The New England Journal of Medicine·D B CinesS Tannenbaum
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J EdgellJ B Graham
Oct 29, 1982·Biochemical and Biophysical Research Communications·C B Underhill
Oct 16, 1980·The New England Journal of Medicine·W R Bell, R M Royall
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Aug 1, 1995·Immunology Today·P YouinouY Shoenfeld
Nov 10, 1994·Journal of Immunological Methods·M N HylkemaR J Smeenk
Aug 7, 1993·Lancet·G R Hughes
Jun 1, 1993·Clinical Immunology and Immunopathology·S ShibataH Fillit
Oct 1, 1996·Clinical Immunology and Immunopathology·H FillitA Dimitriu-Bona
Feb 1, 1997·The American Journal of Medicine·K V SalojinP Y Youinou

❮ Previous
Next ❯

Citations

Jan 17, 2008·Clinical Reviews in Allergy & Immunology·Yves RenaudineauPierre Youinou
Jul 10, 2003·Autoimmunity Reviews·Yves RenaudineauPierre Youinou
Dec 31, 2002·Pathologie-biologie·Joëlle Goetz
Nov 26, 2004·American Journal of Respiratory and Critical Care Medicine·Laimute Taraseviciene-StewartNorbert F Voelkel
Apr 21, 2009·Metabolism: Clinical and Experimental·Mark B ZimeringUNKNOWN Investigators for the VADT
Jun 28, 2006·Arthritis and Rheumatism·Sunil Kumar ChauhanSoniya Nityanand
Dec 6, 2005·Immunobiology·Pierre Youinou
Feb 18, 2006·Autoimmunity Reviews·Pierre YouinouChristophe Jamin
Jul 20, 2002·Journal of Autoimmunity·Karen MaehnssHilmar Lemke
Feb 5, 2021·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Harry N Magnani
Mar 6, 1999·Clinical and Diagnostic Laboratory Immunology·Y RenaudineauP Youinou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.