Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression

Journal of Immunological Methods
Eliane Foulon, Gilles Foucras

Abstract

Whereas studies on dendritic cells in rodents rely largely on bone marrow-derived dendritic cells (BM-DCs), no data are available about BM-DCs in sheep, a species that is largely used for immunology and transplantation studies. We have developed a culture protocol to produce ovine BM-DCs, using 6x(His)-tagged recombinant GM-CSF which was purified from baculovirus-infected insect cells. When ovine bone marrow progenitors were cultured in the presence of recombinant GM-CSF, large numbers of CD11c-positive cells were generated after 6-7 days. The phenotypic appearance of BM-DCs was assessed by flow cytometry and electron microscopy. Two DC subsets were identified that expressed different levels of MHC class II molecules, differed in receptor-mediated endocytosis, and could be separated on CD11b expression. When separated cells were incubated with microbial products, they react differently to those that are considered the TLR2 and TLR4 agonists in other species. Indeed, although CD11b(int/hi) cells were partially resistant to maturation induced by lipoteichoic acid or lipopolysaccharide, MHC class II upregulation was observed on CD11b(dull) cells. Moreover, these cells had strong stimulatory capacity for CD4 T cells when assayed in...Continue Reading

References

Mar 27, 2002·Nature Reviews. Immunology·Ken Shortman, Yong-Jun Liu
Feb 15, 2003·Veterinary Immunology and Immunopathology·E JohanssonC Fossum
Jun 16, 2004·Journal of Leukocyte Biology·Mathieu EpardaudIsabelle Schwartz-Cornil
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May 9, 2006·Nature Immunology·Shalin H NaikKen Shortman

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Citations

Mar 13, 2013·Veterinary Immunology and Immunopathology·Pascale QuéréIsabelle Dimier-Poisson
Jul 25, 2014·Journal of Immunology Research·Afonso P Basto, Alexandre Leitão
Nov 12, 2014·Annual Review of Animal Biosciences·Artur SummerfieldMeret Ricklin
Apr 30, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cyril SeilletSophie Laffont
Mar 17, 2020·Frontiers in Immunology·Robin H G A van den BiggelaarChristine A Jansen

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