Two Populations of Viral Minichromosomes Are Present in a Geminivirus-Infected Plant Showing Symptom Remission (Recovery)

Journal of Virology
Esther Adriana Ceniceros-OjedaR F Rivera-Bustamante

Abstract

Geminiviruses are important plant pathogens characterized by circular, single-stranded DNA (ssDNA) genomes. However, in the nuclei of infected cells, viral double-stranded DNA (dsDNA) associates with host histones to form a minichromosome. In phloem-limited geminiviruses, the characterization of viral minichromosomes is hindered by the low concentration of recovered complexes due to the small number of infected cells. Nevertheless, geminiviruses are both inducers and targets of the host posttranscriptional gene silencing (PTGS) and transcriptional gene silencing (TGS) machinery. We have previously characterized a "recovery" phenomenon observed in pepper plants infected with pepper golden mosaic virus (PepGMV) that is associated with a reduction of viral DNA and RNA levels, the presence of virus-related siRNAs, and an increase in the levels of viral DNA methylation. Initial micrococcal nuclease-based assays pinpointed the presence of different viral chromatin complexes in symptomatic and recovered tissues. Using the pepper-PepGMV system, we developed a methodology to obtain a viral minichromosome-enriched fraction that does not disturb the basic chromatin structural integrity, as evaluated by the detection of core histones. Usin...Continue Reading

References

Jan 5, 1988·Journal of Molecular Biology·T J RichmondR T Simpson
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O VoinnetD C Baulcombe
Feb 13, 2001·Virology·W ZhangR McKenna
May 9, 2003·Nature·Timothy J Richmond, Curt A Davey
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Mark SeemanpillaiAli Rezaian
Jun 17, 2006·Molecular Plant-microbe Interactions : MPMI·Xue-Yu BianM Ali Rezaian
Jul 4, 2008·Journal of Virology·Priya RajaDavid M Bisaro
Sep 13, 2008·BMC Bioinformatics·Eyal GruntmanRavi Sachidanandam
Oct 24, 2008·Phytopathology·Jimena Carrillo-TrippRafael F Rivera-Bustamante
Mar 24, 2009·The Journal of Infectious Diseases·Perumal VivekanandanMichael Torbenson
Jan 19, 2010·Biochimica Et Biophysica Acta·Priya RajaDavid M Bisaro
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hiroaki TachiwanaHitoshi Kurumizaka
Jul 28, 2010·PLoS Pathogens·Zsolt TothJae U Jung
Oct 7, 2010·Nucleic Acids Research·Katsunobu KashiwagiYasufumi Kaneda
Feb 16, 2011·Cell Research·Andrew J Bannister, Tony Kouzarides
Mar 10, 2011·Virology Journal·Ilenia Rentería-CanettRafael F Rivera-Bustamante
May 4, 2011·Reviews in Medical Virology·Michael NevelsChristina Paulus
Aug 13, 2011·Journal of Virology·T PaprotkaH Jeske
Jun 21, 2012·Molecular Plant-microbe Interactions : MPMI·Ana P LunaEduardo R Bejarano
Aug 22, 2012·Frontiers in Plant Science·Jean-Sébastien ParentHervé Vaucheret
Jun 4, 2013·Biochemical and Biophysical Research Communications·Hyun Kyung ParkJin-Wook Kim
Jul 28, 2013·International Journal of Molecular Sciences·Mikhail M Pooggin
Oct 17, 2013·Nature Reviews. Microbiology·Nathan Pumplin, Olivier Voinnet
Jan 28, 2014·Archives of Virology·Arvind VarsaniJudith K Brown
Mar 4, 2014·Annual Review of Plant Biology·Nicolas G Bologna, Olivier Voinnet
Mar 4, 2014·Biochimica Et Biophysica Acta·Sarah G Swygert, Craig L Peterson
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cheng QinZhiming Zhang

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Citations

Oct 8, 2016·Virology·Kathrin DeuschleHolger Jeske
Jun 8, 2018·Journal of Virology·Tami CourseyDavid M Bisaro
Jun 19, 2019·Molecular Plant Pathology·Prabu GnanasekaranSupriya Chakraborty
Sep 12, 2018·Viruses·Hernan Garcia-Ruiz
Jul 10, 2019·Molecular Plant Pathology·Hernan Garcia-Ruiz
Mar 11, 2020·Viruses·Myriam G Rodríguez-GandarillaRafael F Rivera-Bustamante
Sep 2, 2020·Journal of Experimental Botany·Fauzia Zarreen, Supriya Chakraborty
Jan 9, 2021·Applied Microbiology and Biotechnology·Saumik BasuSupriya Chakraborty
Apr 27, 2021·Frontiers in Microbiology·Karuppannan Veluthambi, Sukumaran Sunitha
May 1, 2021·Viruses·Rosalba Rodriguez-PeñaHernan Garcia-Ruiz
Jul 4, 2021·Trends in Microbiology·Ragunathan DevendranSupriya Chakraborty
Jul 11, 2021·Virology Journal·Neha GuptaSupriya Chakraborty

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