Two pore domain potassium channels in cerebral ischemia: a focus on K2P9.1 (TASK3, KCNK9).

Experimental & Translational Stroke Medicine
Petra EhlingSven G Meuth

Abstract

Recently, members of the two-pore domain potassium channel family (K2P channels) could be shown to be involved in mechanisms contributing to neuronal damage after cerebral ischemia. K2P3.1-/- animals showed larger infarct volumes and a worse functional outcome following experimentally induced ischemic stroke. Here, we question the role of the closely related K2P channel K2P9.1. We combine electrophysiological recordings in brain-slice preparations of wildtype and K2P9.1-/- mice with an in vivo model of cerebral ischemia (transient middle cerebral artery occlusion (tMCAO)) to depict a functional impact of K2P9.1 in stroke formation. Patch-clamp recordings reveal that currents mediated through K2P9.1 can be obtained in slice preparations of the dorsal lateral geniculate nucleus (dLGN) as a model of central nervous relay neurons. Current characteristics are indicative of K2P9.1 as they display an increase upon removal of extracellular divalent cations, an outward rectification and a reversal potential close to the potassium equilibrium potential. Lowering extracellular pH values from 7.35 to 6.0 showed comparable current reductions in neurons from wildtype and K2P9.1-/- mice (68.31 +/- 9.80% and 69.92 +/- 11.65%, respectively). Th...Continue Reading

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Citations

Nov 16, 2011·Pflügers Archiv : European journal of physiology·Pawan BistaThomas Budde
Feb 5, 2013·Experimental & Translational Stroke Medicine·Petra EhlingSven G Meuth
Oct 19, 2010·Trends in Pharmacological Sciences·Zeineb Es-Salah-LamoureuxDavid Fedida
Oct 28, 2014·Pflügers Archiv : European journal of physiology·Pawan BistaThomas Budde
Dec 9, 2014·Pflügers Archiv : European journal of physiology·Petra EhlingStefan Bittner
Mar 21, 2015·Pflügers Archiv : European journal of physiology·Vijay ReniguntaJürgen Daut
Oct 16, 2014·Pflügers Archiv : European journal of physiology·Eva GöbChristoph Kleinschnitz
Dec 16, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Khodadad NamiranianRobert M Bryan
Dec 18, 2013·Molecular Pharmacology·Emma L VealeAlistair Mathie

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GraphPad
HEKA Elektronik
PrismGraph
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