Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats

Frontiers in Zoology
Xiao GuoJian-Xu Zhang

Abstract

In rats, urine-borne male pheromones comprise organic volatile compounds and major urinary proteins (MUPs). A number of volatile pheromones have been reported, but no MUP pheromones have been identified in rat urine. We used sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing electrophoresis (IEF), nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) after in gel digestion of the proteins and quantitative real-time PCR (qRT-PCR) and showed that the levels of two MUPs, odorant-binding protein 3 (OBP3) (i.e. PGCL4) and MUP13 (i.e. PGCL1), in urine and their mRNAs in liver were higher in males than in females and were suppressed by orchidectomy and restored by testosterone treatment (T treatment). We then generated recombinant MUPs (rMUPs) and found that the sexual attractiveness of urine from castrated males to females significantly increased after the addition of either recombinant OBP3 (rOBP3) or recombinant MUP13 (rMUP13). Using c-Fos immunohistochemistry, we further examined neuronal activation in the brains of female rats after they sniffed rOBP3 or rMUP13. Both rOBP3 and rMUP13 activated the accessory olfactory bulb (AOB), medial preoptic area (MPA), bed nucleus of the stri...Continue Reading

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Citations

Jul 26, 2019·Scientific Reports·Guadalupe Gómez-BaenaRobert J Beynon
Jan 15, 2021·The Journal of Clinical Investigation·Pirunthan PerampalamFrederick A Dick

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Datasets Mentioned

BETA
PXD008341

Methods Mentioned

BETA
electrophoresis
PCR
protein assay
urine collections

Software Mentioned

Proteome Discoverer
Primer
Sequest
Image
DANMAN
ExPASy
Compute
Pro Plus
BLAST
SPSS

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