Two primitive neuroectodermal tumor cell lines require an activated insulin-like growth factor I receptor for growth in vitro

Neurosurgery
L S ChinC Raffel

Abstract

To determine the expression of the insulin-like growth factors (IGFs) and the IGF-I receptor in primitive neuroectodermal tumor cell lines and to assess the importance of these proteins in the growth of cell lines in vitro. Ribonucleic acid blotting and reverse transcriptase-polymerase chain reaction were used for detection of IGF and IGF-I expression. Ribonucleic acid blotting was used for detection of up-regulation of c-fos in the presence of exogenous growth factor. Immunoprecipitation was used to demonstrate autophosphorylation of the receptor in the presence of exogenous growth factor. Ligand binding analysis was used to determine the binding affinity of the receptor and the number of receptors per cell. Growth of curves in the presence of monoclonal antibody that blocks binding of ligand to receptor was measured to determine the requirement for an activated receptor during growth. Expression of IGF-II was identified in one cell line. No expression of IGF-I was seen in any cell line. Expression of IGF-I receptor was detected in all three cell lines. Immunoprecipitation experiments demonstrated autophosphorylation of the receptor after addition of IGF-I to growing cells. Ligand binding analysis revealed 9.2 x 10(4) and 4 x ...Continue Reading

References

May 1, 1992·Journal of Neuropathology and Experimental Neurology·D FultsR D McKay
Apr 15, 1992·Journal of the National Cancer Institute·J G ReeveN M Bleehen
Dec 1, 1988·The Journal of Clinical Endocrinology and Metabolism·W H BusbyD R Clemmons
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S MohanD J Baylink
Nov 30, 1989·Biochemical and Biophysical Research Communications·M ShimonakaN Ling
Jan 1, 1989·Progress in Growth Factor Research·R C Baxter, J L Martin
Sep 1, 1989·The Journal of Clinical Investigation·O M El-BadryM A Israel
Jun 1, 1987·The Journal of Clinical Endocrinology and Metabolism·R W FurlanettoC Wisehart
Dec 1, 1987·Journal of Cellular Physiology·C A ConoverR L Hintz
Sep 1, 1985·Journal of Neuropathology and Experimental Neurology·P F JacobsenJ M Papadimitriou
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P RotweinJ E Krause
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F A McMorrisR W Furlanetto
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J F MillD N Ishii
Nov 1, 1985·Journal of Neuropathology and Experimental Neurology·H S FriedmanD D Bigner
Mar 1, 1984·Clinics in Endocrinology and Metabolism·J ZapfE R Froesch
Jun 1, 1983·Human Pathology·L E Becker, D Hinton
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 10, 1984·Nature·A E ReeveL J Millow
Dec 8, 1983·Nature·M JansenJ L Van den Brande
Aug 30, 1984·Biochemical and Biophysical Research Communications·J BlattT P Foley

❮ Previous
Next ❯

Citations

Mar 7, 2000·The American Journal of Pathology·E de AlavaM Ladanyi
Jul 17, 2004·Neoplasia : an International Journal for Oncology Research·Corey Raffel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.