Two proximal activating protein-1-binding sites are sufficient to stimulate transcription of the ovine follicle-stimulating hormone-beta gene

Endocrinology
B D StrahlW L Miller

Abstract

FSH is an important regulator of mammalian gametogenesis and the female reproductive cycle. Although little is known about the transcriptional regulation of the beta-subunit (the rate-limiting subunit of FSH synthesis), sequence analysis of the ovine FSHbeta promoter has revealed a number of potential activating protein-1 (AP-1; Jun/Fos)-binding sites. To determine whether the gene encoding the beta-subunit of ovine FSH (oFSHbeta) is responsive to AP-1 transcriptional complexes, chimeric constructs containing deleted portions of the oFSHbeta promoter fused to a luciferase reporter were transiently transfected along with c-Jun and c-Fos expression constructs into JAR cells. Analysis of these deletion constructs revealed that the proximal promoter of oFSHbeta is highly stimulated by c-Jun and c-Fos proteins (typically 20-fold with a reporter construct containing oFSHbeta sequences from -215 to +759 bp). This stimulation was lost when a similar construct containing sequences from -84 to +759 bp was tested. Transcriptional start site analysis using reverse transcription-PCR verified that the transcriptional initiation of the -215-bp deletion construct, with or without cotransfected c-Jun/c-Fos, was the same as that observed in vivo...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R S FrilingV Daniel
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T YoungM J Iadarola
Feb 1, 1990·Endocrine Reviews·S D GharibW W Chin
Dec 22, 1989·Science·J L SonnenbergT Curran
Jan 1, 1988·International Urology and Nephrology·J SzöllösiM Sas
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jul 1, 1970·Archives of Biochemistry and Biophysics·W E Braselton, W H McShan
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Nov 1, 1993·Endocrinology·M HashimotoW Vale

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Citations

Jun 28, 2011·Molecular Endocrinology·Nicholas J JusticeLouise M Bilezikjian
Jan 1, 2013·Molecular Endocrinology·Gaddameedi R ReddyDjurdjica Coss
Apr 21, 2010·Fertility and Sterility·Daniel J BernardPankaj Lamba
Oct 12, 2004·Biochemical and Biophysical Research Communications·Satoko AikawaYukio Kato
Feb 14, 2018·Journal of Molecular Endocrinology·Nandana Das, T Rajendra Kumar
May 6, 2015·Molecular Endocrinology·Jérôme FortinDaniel J Bernard
Jan 1, 2008·Gene Regulation and Systems Biology·Masanori Yoshida
May 1, 2021·Animals : an Open Access Journal From MDPI·Hao-Qi WangJia-Bao Zhang

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