Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004)

Journal of Clinical Microbiology
K LoensM Ieven

Abstract

The quality performance of laboratories for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae by two quality control (QC) exercises with a 2-year interval was investigated. For the 2002 QC exercise, specimens were spiked with M. pneumoniae at concentrations of 5,000, 500, 50, and 0 color-changing units (CCU)/100 microl. The limit of detectability was 50 CCU/100 microl. Therefore, this concentration was omitted from the 2004 panel and was excluded from the analysis. In 2002, 2 out of 12 participants obtained 100% correct results, 2 out of 12 produced false-positive results, and 10 out of 12 had between 0 out of 9 and 8 out of 9 correct positive results. In 2004, correct results were obtained in 15 out of 18 tests, and no false-positive results were reported. In 2002, specimens were spiked with C. pneumoniae at concentrations of 490, 49, 4.9, and 0 inclusion-forming units/100 microl (IFU/100 microl). In the 2004 panel, samples spiked with a lower dilution of 0.49 IFU/100 microl were added to the panel. For the C. pneumoniae QC, correct results were produced in 12 out of 16 and 13 out of 18 tests in 2002 and in 2004, respectively. Both multiplex PCR and nucleic acid sequence-based amplification (NASBA) formats sc...Continue Reading

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Citations

Jun 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K LoensM Ieven
Jan 29, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N ReintonA Moghaddam
Jan 24, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Swati Kumar, Margaret R Hammerschlag
Jun 29, 2007·The Pediatric Infectious Disease Journal·Margaret R Hammerschlag
Apr 11, 2008·Journal of Clinical Microbiology·Patrick BastienUdo Reischl
Oct 18, 2011·Emerging Infectious Diseases·Roxanne E StrachanUNKNOWN Australian Research Network in Empyema
Sep 29, 2009·European Journal of Internal Medicine·Georgios S PapaetisDora Orphanidou
Nov 3, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Margareta Ieven
Nov 21, 2013·The Lancet Infectious Diseases·Ramanan LaxminarayanOtto Cars
May 26, 2017·Clinical Microbiology Reviews·Ken B WaitesT Prescott Atkinson
Feb 3, 2007·Expert Review of Anti-infective Therapy·Guy Vernet
Feb 23, 2007·Journal of Medical Microbiology·Andrei KutlinMargaret R Hammerschlag

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