Two rat mononuclear cell-derived slow reacting substances: kinetic evidence that the peripheral airways-selective spasmogen is derived from a nonselectively acting precursor

Immunopharmacology
M K BachJ M Drazen

Abstract

The purpose of this study was to extend our knowledge of two slow reacting substances that are formed in rat mononuclear cells upon stimulation by the ionophore A 23187. Slow reacting substance (SRS) preparations were further purified through the sequential high performance liquid chromatographic procedures starting with exudates from cells that had been prelabeled with tritiated arachidonate. Approximately 2% of the released radioactivity (0.3% of the radioactivity that was taken up by the cells) cochromatographed with biologic activity in both preparations. Kinetic studies revealed that one of the SRSs was formed more rapidly than the other and analysis by nonlinear computer modeling techniques suggests a possible product-precursor relationship between the two substances. Activity of the preparations on guinea pig lung parenchyma and trachea was compared using a histamine standard; only the more slowly formed substance had the selective activity on the parenchyma that we associate with slow reacting substance of anaphylaxis (SRS-A). Taken together, these findings suggest that a material which may be a precursor of SRS-A, if it is also formed and accumulated in vivo, may contribute to the more central airway constriction which...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C MurphyB Samuelsson
Dec 28, 1979·Biochemical and Biophysical Research Communications·S HammarströmE J Corey
Jan 1, 1979·The Journal of Allergy and Clinical Immunology·C W Parker
Feb 15, 1979·European Journal of Pharmacology·H W Mitchell, M A Denborough
Aug 28, 1979·Biochemical and Biophysical Research Communications·C W ParkerS F Falkenhein
Oct 17, 1973·Nature: New Biology·J AugsteinM L Tattersall
Feb 12, 1980·Biochemical and Biophysical Research Communications·S HammarströmE J Corey
Apr 29, 1980·Biochemical and Biophysical Research Communications·M K BachB Samuelsson
Jun 30, 1980·Biochemical and Biophysical Research Communications·D A ClarkB Samuelsson
Jun 1, 1965·The Journal of Pharmacy and Pharmacology·J W CONSTANTINE

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Apr 29, 1980·Biochemical and Biophysical Research Communications·M K BachB Samuelsson

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Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.