Two ResD-controlled promoters regulate ctaA expression in Bacillus subtilis

Journal of Bacteriology
Salbi PaulF Marion Hulett

Abstract

The Bacillus subtilis ResDE two-component system plays a positive role in global regulation of genes involved in aerobic and anaerobic respiration. ctaA is one of the several genes involved in aerobic respiration that requires ResD for in vivo expression. The ctaAB-divergent promoter regulatory region has three ResD binding sites; A1, A2, and A3. The A2 site is essential for in vivo promoter activity, while binding sites A2 and A3 are required for full ctaA promoter activity. In this study, we demonstrate the role of ResD~P in the activation of the ctaA promoter using an in vitro transcription system. The results indicate that the ctaA promoter (binding sites A2 and A3) has two transcriptional start sites. Binding site A2 was sufficient for weak transcription of the upstream promoter (Pv) by Esigma(A), transcription which was enhanced approximately 1.5-fold by ResD and 5-fold by ResD~P. The downstream promoter (Ps) required both binding sites A2 and A3 and was not transcribed by Esigma(A) with or without ResD~P. RNA polymerase (RNAP) isolated from B. subtilis when cells were at the end of exponential growth (T(0)) or 3, 4, or 5 h into the stationary phase (T(3), T(4), or T( 5), respectively) was used in in vitro transcription a...Continue Reading

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Citations

Jul 3, 2004·Journal of Bacteriology·Matthew SchauF Marion Hulett
Sep 18, 2004·Journal of Bacteriology·Elisabeth HärtigMichiko M Nakano
Feb 3, 2006·Journal of Bacteriology·Ankita Puri-TanejaF Marion Hulett
Jul 11, 2006·Journal of Molecular Microbiology and Biotechnology·Soo-Keun Choi, Milton H Saier
Oct 6, 2010·Genome Biology·Peter WaltmanRichard Bonneau

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