Two sporulation-specific chitin deacetylase-encoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae.

The Journal of Biological Chemistry
A ChristodoulidouG Thireos

Abstract

The formation of the ascospore wall of Saccharomyces cerevisiae requires the coordinate activity of enzymes involved in the biosynthesis of its components such as chitosan, the deacetylated form of chitin. We have cloned the CDA1 and CDA2 genes which together account for the total chitin deacetylase activity of the organism. We have shown that expression of these genes is restricted to a distinct time period during sporulation. The two genes are functionally redundant, each contributing equally to the total chitin deacetylase activity. Diploids disrupted for both genes sporulate as efficiently as wild type cells, and the resulting mutant spores are viable under standard laboratory conditions. However, they fail to emit the natural fluorescence of yeast spores imparted by the dityrosine residues of the outermost ascospore wall layer. Moreover, mutant spores are relatively sensitive to hydrolytic enzymes, ether, and heat shock, a fact that underscores the importance of the CDA genes for the proper formation of the ascospore wall.

References

Jun 16, 1975·European Journal of Biochemistry·Y Araki, E Ito
Apr 27, 1978·Archives of Microbiology·N J Kreger-Van Rij
Jul 1, 1991·The Journal of Cell Biology·M H ValdiviesoA Durán
Jul 1, 1991·The Journal of Cell Biology·J A ShawE Cabib
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Feb 4, 1974·Biochemical and Biophysical Research Communications·Y Araki, E Ito
Dec 21, 1984·Nucleic Acids Research·I TörökA Kondorosi
Aug 1, 1984·Journal of General Microbiology·L L Davis, S Bartnicki-Garcia
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Sep 1, 1995·Microbiological Reviews·V J CidM Sánchez
Nov 3, 1995·The Journal of Biological Chemistry·I Tsigos, V Bouriotis
Feb 1, 1995·Journal of Biochemistry·X D GaoK Onodera
Jul 1, 1994·Yeast·F M Klis
Mar 1, 1994·Microbiological Reviews·A P Mitchell
Jan 1, 1993·Annual Review of Microbiology·C E Bulawa
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D KafetzopoulosV Bouriotis
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M JohnJ Schell
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D KafetzopoulosV Bouriotis
Mar 26, 1962·Biochimica Et Biophysica Acta·S BARTNICKI-GARCIA, W J NICKERSON
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C E BallouW L Whelan

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Citations

Jun 17, 2000·Trends in Biotechnology·I TsigosV Bouriotis
Dec 13, 2000·Microbes and Infection·H B DeisingM Wernitz
Jan 13, 2000·Molecular Microbiology·M ArellanoM Henar Valdivieso
Dec 14, 2004·Eukaryotic Cell·Alison ColuccioAaron M Neiman
Mar 10, 2005·Eukaryotic Cell·Michelle A IwamotoJoanne Engebrecht
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Aaron M Neiman
Aug 21, 2012·PloS One·Ulrich SchlechtRonald W Davis
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M LevitzC A Specht
Mar 16, 2013·Critical Reviews in Microbiology·Surinder Kaur, Gurpreet Singh Dhillon
Oct 20, 2012·Critical Reviews in Biotechnology·Gurpreet Singh DhillonMausam Verma
May 28, 2009·Protein Science : a Publication of the Protein Society·Jonathan E UrchDaan M F van Aalten
Nov 24, 2004·Protein Expression and Purification·Binesh ShresthaFrançoise L Hegarat
Jan 29, 2002·Protein Expression and Purification·Aggeliki MartinouVassilis Bouriotis
Nov 7, 2009·FEMS Yeast Research·José Ruiz-Herrera, Lucila Ortiz-Castellanos
Mar 4, 2008·Protein Expression and Purification·Carole GauthierMarie-France Jaspar-Versali
Dec 7, 2017·Journal of Biochemistry·Hua-Ping PanHideki Nakanishi
Jan 27, 2018·Journal of Fungi·Kai ZhangAaron M Neiman
Jul 1, 2017·Microbiology and Molecular Biology Reviews : MMBR·Marco AgostoniMichael A Marletta
May 20, 2014·Applied and Environmental Microbiology·Libing ShiHideki Nakanishi
Jun 8, 2014·Applied and Environmental Microbiology·Haini ZhangHideki Nakanishi
Oct 30, 2016·Applied and Environmental Microbiology·Shoa NaqviBruno M Moerschbacher
Nov 29, 2001·The Journal of Cell Biology·H TachikawaA M Neiman
Sep 7, 2019·Current Protein & Peptide Science·Verónica PlazaLuis Castillo
Aug 11, 2017·The Journal of Biological Chemistry·Leo D BemenaHideki Nakanishi
May 13, 2017·Scientific Reports·Zhanliang LiuVincent G H Eijsink

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