Two-stage oxygen supply strategy based on energy metabolism analysis for improving acetic acid production by Acetobacter pasteurianus

Journal of Industrial Microbiology & Biotechnology
Yu ZhengMin Wang

Abstract

Oxygen acts as the electron acceptor to oxidize ethanol by acetic acid bacteria during acetic acid fermentation. In this study, the energy release rate from ethanol and glucose under different aerate rate were compared, and the relationship between energy metabolism and acetic acid fermentation was analyzed. The results imply that proper oxygen supply can maintain the reasonable energy metabolism and cell tolerance to improve the acetic acid fermentation. Further, the transcriptions of genes that involve in the ethanol oxidation, TCA cycle, ATP synthesis and tolerance protein expression were analyzed to outline the effect of oxygen supply on cell metabolism of Acetobacter pasteurianus. Under the direction of energy metabolism framework a rational two-stage oxygen supply strategy was established to release the power consumption and substrates volatilization during acetic acid fermentation. As a result, the acetic acid production rate of 1.86 g/L/h was obtained, which were 20.78% higher than that of 0.1 vvm one-stage aerate rate. And the final acetic acid concentration and the stoichiometric yield were 88.5 g/L and 94.1%, respectively, which were 84.6 g/L and 89.5% for 0.15 vvm one-stage aerate rate.

References

Apr 1, 1990·Journal of Bacteriology·M FukayaT Beppu
Jun 22, 2005·Journal of Bacteriology·Kazunobu MatsushitaHirohide Toyama
Sep 1, 2005·Applied Microbiology and Biotechnology·Janja TrcekKazunobu Matsushita
Oct 20, 2005·Journal of Bioscience and Bioengineering·Akiko Okamoto-KainumaYukimichi Koizumi
Dec 14, 2011·Journal of Proteomics·Cristina Andrés-BarraoFrançois Barja
Aug 21, 2012·Journal of Bioscience and Bioengineering·Kenta SakuraiHiroyuki Arai
Jan 16, 2014·Indian Journal of Microbiology·Dhouha Mamlouk, Maria Gullo
May 20, 2014·Applied and Environmental Microbiology·Philipp AdlerChristoph Wittmann
Dec 9, 2014·Biotechnology Advances·Natsaran SaichanaJitka Frebortova
Jan 13, 2015·World Journal of Microbiology & Biotechnology·Bin WangFusheng Chen
Jul 5, 2015·Applied Microbiology and Biotechnology·Janja TrčekLaura R Jarboe
Nov 2, 2016·Journal of Industrial Microbiology & Biotechnology·George N Bennett, Ka-Yiu San
Feb 13, 2018·Applied Microbiology and Biotechnology·Yu ZhengMin Wang

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Methods Mentioned

BETA
PCR
biosensor

Software Mentioned

Origin Pro
SPSS
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