Two-step gene transfer using an adenoviral vector carrying the CD4 gene and human immunodeficiency viral vectors

Human Gene Therapy
K MiyakeT Shimada

Abstract

Human immunodeficiency virus-1 (HIV-1) belongs to the lentivirus subfamily of retroviruses and has several interesting features, including T cell tropism and the ability to infect nondividing cells. Replication-incompetent HIV vectors were developed and were shown to be capable of targeted gene transfer into CD4+T cells. This strict T cell tropism may be important for the development of gene therapy of acquired immunodeficiency syndrome (AIDS), but it hampers the use of the HIV vector for other gene transfer applications. To expand the host range of the HIV vector, we established the two-step gene transfer system, which allows us to transduce non-T cells stably. In the first step, the CD4 gene was introduced into target cells using a replication-defective adenoviral vector. Transient but high-level expression of CD4 molecules was detected in both adherent and floating cells. In the subsequent step, the cells were incubated with HIV vectors. Stable integration of the HIV vector was demonstrated in cells transduced with the adenoviral vector. These results indicate that transient expression of CD4 molecules by the adenoviral vector is sufficient to render non-T cells susceptible to HIV-mediated gene transfer. This two-step gene t...Continue Reading

References

Dec 1, 1991·The Journal of Experimental Medicine·J B WeinbergM H Malim
Jan 24, 1985·Nature·L RatnerK Baumeister
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S F YuE Gilboa
Oct 1, 1994·Human Gene Therapy·R A BrodskyA W Nienhuis
Jun 1, 1994·Japanese Journal of Medical Science & Biology·Y KanegaeI Saito
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G MillerA D Miller
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M van ZeijlB O'Hara
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S MiyakeI Saito

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Citations

Aug 16, 2001·International Journal of Hematology·Noriyasu SakaiTakashi Shimada
Feb 12, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Y KitamuraK Yoshiike
Feb 8, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shizuya SaikaAkira Ooshima
Jul 20, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shizuya SaikaAnita B Roberts
Apr 25, 2012·Archives of Virology·Duo-Ling ChenTao Hung
Jul 14, 2000·The Journal of Gene Medicine·M H ZahlerR G Pestell
Aug 30, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nobuhiro IzumiKazuo Ikeda

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