Two tandem binding sites for sterol regulatory element binding proteins are required for sterol regulation of fatty-acid synthase promoter.

The Journal of Biological Chemistry
M M Magaña, T F Osborne

Abstract

We previously reported that sterol regulation of the rat fatty-acid synthase was lost when the DNA sequence between -73 and -43 of the promoter was deleted from a luciferase reporter construct (Bennett, M. K., Lopez, J. M., Sanchez, H. B., and Osborne, T. F. (1995) J. Biol. Chem. 270, 25578-25583). We also showed that there was a binding site for sterol regulatory element binding protein-1 (SREBP-1) in this region that contains a palindromic E-box motif (5'-CANNTG-3'). This is the consensus recognition element for basic-helix-loop-helix leucine zipper containing proteins such as the SREBPs. However, the SREBPs are unique basic-helix-loop-helix leucine zipper proteins that not only bind to a subset of E-boxes but also to the direct repeat SRE-1 element of the low density lipoprotein receptor promoter as well as to variant sites present in the promoters for key enzymes of both cholesterol and fatty acid biosynthesis. Based on the sequence of the variant SREBP recognition sites in these other promoters, we noted there was more than one potential recognition site for SREBP within the -73 to -43 interval of the fatty-acid synthase promoter. In the present studies we have systematically mutated these potential SREBP sites and have an...Continue Reading

References

Nov 23, 1990·Science·T K BlackwellH Weintraub
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T F OsborneJ L Goldstein
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Sep 15, 1995·The Journal of Biological Chemistry·G GuanI Shechter
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z ShengJ L Goldstein
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X HuaX Wang
Jun 21, 1994·Biochimica Et Biophysica Acta·C MurreM H Stuiver
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J EricssonP A Edwards
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M LopezT F Osborne
Jan 1, 1995·Critical Reviews in Eukaryotic Gene Expression·T F Osborne

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May 23, 2001·Archives of Biochemistry and Biophysics·S S WolfM Schweizer
May 1, 2009·European Journal of Nutrition·Heather E RasmussenJi-Young Lee
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Jun 23, 2010·In Vitro Cellular & Developmental Biology. Animal·Jiakai Wu, Alan J Dickson
Jul 31, 2002·Biochemical and Biophysical Research Communications·Takayuki Y NaraManabu T Nakamura
Jan 28, 2003·Progress in Lipid Research·David Y Hui
Dec 5, 2003·Progress in Lipid Research·Rosalind A Coleman, Douglas P Lee
Mar 1, 1997·Progress in Lipid Research·C F Semenkovich
May 4, 2000·Progress in Lipid Research·N D RidgwayM K Storey
Aug 2, 2003·Molecular and Cellular Endocrinology·Hannelore HeemersJohannes V Swinnen
Jan 23, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M T Nakamura, T Y Nara
Dec 9, 2000·Biochimica Et Biophysica Acta·P A EdwardsA Venkateswaran
Dec 4, 2001·Biochimica Et Biophysica Acta·T SugawaraT Ishibashi
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Arun J Sanyal
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Megumi HatoriYoshitaka Fukada
May 18, 1999·Current Opinion in Lipidology·B E AouizeratA J Lusis
May 8, 2008·Current Opinion in Lipidology·Pierre-Damien DenechaudCatherine Postic
May 4, 2010·Eukaryotic Cell·Ari RosenbachCarol A Kumamoto
Jun 17, 2009·Molecular and Cellular Biology·Erinn B RankinVolker H Haase
Sep 5, 2001·Molecular and Cellular Biology· Vik A, J Rine
Mar 16, 2006·Molecular and Cellular Biology·Bridget L ToddPeter J Espenshade
Jun 29, 2000·Annual Review of Biochemistry·P A Edwards, J Ericsson
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Aug 15, 2000·Annual Review of Nutrition·S M Rangwala, M A Lazar
Jun 11, 2004·Annual Review of Nutrition·Manabu T Nakamura, Takayuki Y Nara
Apr 13, 2007·Annual Review of Nutrition·Catherine PosticJean Girard

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