Two-Tier Lyme Disease Serology Test Results Can Vary According to the Specific First-Tier Test Used

Journal of the Pediatric Infectious Diseases Society
Alexandra B MauldenLise E Nigrovic

Abstract

Variability in 2-tier Lyme disease test results according to the specific first-tier enzyme immunoassay (EIA) in children has not been examined rigorously. In this study, we compared paired results of clinical 2-tier Lyme disease tests to those of the C6 peptide EIA followed by supplemental immunoblotting (C6 2-tier test). We performed a prospective cohort study of children aged ≥1 to ≤21 years who were undergoing evaluation for Lyme disease in the emergency department at 1 of 6 centers located in regions in which Lyme disease is endemic. The clinical first-tier test and a C6 EIA were performed on the same serum sample with supplemental immunoblotting if the first-tier test result was either positive or equivocal. We compared the results of the paired clinical and C6 2-tier Lyme disease test results using the McNemar test. Of the 1714 children enrolled, we collected a research serum sample from 1584 (92.4%). The clinical 2-tier EIA result was positive in 316 (19.9%) children, and the C6 2-tier test result was positive or equivocal in 295 (18.6%) children. The clinical and C6 2-tier test results disagreed more often than they would have by chance alone (P = .002). Of the 39 children with either a positive clinical or C6 2-tier t...Continue Reading

References

Dec 22, 1989·JAMA : the Journal of the American Medical Association·B S SchwartzS I Shahied
Jun 1, 1987·The Journal of Infectious Diseases·C W HedbergK E White
Feb 1, 1985·The American Journal of Medicine·M ShresthaA C Steere
Aug 11, 1995·MMWR. Morbidity and Mortality Weekly Report·UNKNOWN Centers for Disease Control and Prevention (CDC)
Jun 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J L SchmitzJ D Folds
Jul 23, 2003·Statistics in Medicine·Valerie L DurkalskiPhilip F Rust
Jul 16, 2005·Clinical Microbiology Reviews·Maria E Aguero-RosenfeldGary P Wormser
Jun 6, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Allen C SteereVijay K Sikand
Aug 26, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A BrandaMary Jane Ferraro
Mar 1, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V SeriburiG P Wormser
Jun 1, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alison F HinckleyPaul S Mead
Sep 4, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Brian A FallonCarl Brenner
Oct 30, 2014·Current Infectious Disease Reports·Roberta L DeBiasi
Mar 13, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Claudia R MolinsJohn T Belisle
May 10, 2016·The Journal of Pediatrics·Paul M LantosLise E Nigrovic
Jul 1, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Susan C LipsettLise E Nigrovic
Dec 7, 2016·International Journal of General Medicine·Michael J Cook, Basant K Puri
Oct 6, 2017·International Journal of General Medicine·Michael J Cook, Basant K Puri
Nov 28, 2017·Pediatrics·Lise E NigrovicUNKNOWN for Pedi Lyme Net
Dec 12, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John A BrandaSteven E Schutzer

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Citations

Sep 17, 2020·PloS One·Brendan J McMullanUNKNOWN Australian PICNICC study group and the PREDICT network
Mar 13, 2021·The Pediatric Infectious Disease Journal·Aris GarroUNKNOWN for Pedi Lyme Net
Oct 6, 2021·Vector Borne and Zoonotic Diseases·Paul M LantosLise E Nigrovic
Dec 15, 2019·Pediatrics·Lise E NigrovicUNKNOWN PEDI LYME NET
Jun 24, 2021·Pediatric Emergency Care·Duncan S GrantUNKNOWN for Pedi Lyme Net

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