Two toxins from the venom of Trimeresurus tokarensis

The International Journal of Biochemistry
S SekoguchiH Sugihara

Abstract

1. Two toxins, Tokaratoxin-1 (TT-1) and Tokaratoxin-2 (TT-2), were isolated from the venom of Trimeresurus tokarensis using gel filtration on a Sephadex G-100 column, followed by chromatography on DEAE-Sephacel and carboxymethyl-cellulose. TT-1 possessed both hemorrhagic and proteolytic activities. However, TT-2 did not show hemorrhagic activity. 2. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing and SDS-PAGE. 3. Molecular weights of TT-1 and TT-2 were 71,000 and 25,400, respectively. Although TT-2 is a basic protein, TT-1 is an acidic protein. 4. Biological activities of TT-1 and TT-2 were inhibited by EDTA, EGTA and o-phenanthroline, suggesting that the toxins are metalloproteins. Atomic absorption analyses indicated that TT-1 contains 2.79 mol Ca/mol protein and TT-2 contains 1.04 mol Ca/mol protein and 1.07 mol Zn/mol protein, respectively. 5. The two toxins degraded the A alpha and B beta chains of fibrinogen. 6. TT-1 induced necrosis in addition to its hemorrhagic activity while TT-2 induced necrosis only.

References

Dec 1, 1979·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·H SugiharaT Tanaka
Oct 24, 1979·Biochimica Et Biophysica Acta·T Omori-Satoh, S Sadahiro
Nov 1, 1978·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·H SugiharaT Tanaka
Feb 20, 1976·Biochimica Et Biophysica Acta·C Ouyang, C M Teng
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·S HagiharaA T Tu
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·M KishidaH Sugihara
Feb 15, 1973·Biochimica Et Biophysica Acta·A M SpiekermanJ M Prescott
Jan 1, 1982·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·T NikaiH Sugihara
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·H SugiharaT Nikai

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Citations

Dec 1, 1994·The International Journal of Biochemistry·Y KomoriH Sugihara
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·S A WeinsteinL A Smith
Aug 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·M MaruyamaN Nakajima
Jun 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·R F Clark, T M Davidson

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