Two types of galactosylated fucose motifs are present on N-glycans of Haemonchus contortus

Glycobiology
Katharina Paschinger, Iain B H Wilson

Abstract

N-Glycans from the nematode Haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the N,N'-diacetylchitobiosyl core, two being on the reducing-terminal proximal GlcNAc and one on the distal core GlcNAc residue. The assumption was that truncated glycans from this organism with three hexose residues have the composition Man3GlcNAc2Fuc1-3. In this study, we have performed HPLC and MALDI-TOF MS/MS in combination with selected digestions of N-glycans from Haemonchus. A dominant trifucosylated Hex3HexNAc2Fuc3 glycan was modified not only with α1,6-fucose but also with a proximal core α1,3-fucose and a galactosylated distal α1,3-fucose; thereby, only two of the hexose residues were mannose. Other N-glycans displayed galactosylation of the core α1,6-fucose, antennal fucosylation or modification with phosphorylcholine. Thus, the N-glycans of Haemonchus contain a number of potentially immunogenic glycan epitopes also found in other parasites and our proposed structures are in line with the previously defined specificity of nematode glycosyltransferases as we show that distal fucosylation and the presence of an α1,6-mannose are apparen...Continue Reading

References

May 15, 1988·Analytical Biochemistry·N TomiyaN Takahashi
Jan 1, 1996·Parasitology Research·H D Schallig, M A van Leeuwen
Jul 14, 1998·Molecular and Biochemical Parasitology·S M HaslamA Dell
Aug 13, 2005·Veterinary Parasitology·W R Hein, G B L Harrison
Jan 30, 2008·Carbohydrate Research·Katharina PaschingerIain B H Wilson
Oct 11, 2011·Glycobiology·Katharina PaschingerIain B H Wilson
Sep 17, 2013·Applied Microbiology and Biotechnology·Martin DragositsDubravko Rendic

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Citations

Dec 10, 2015·Analytical and Bioanalytical Chemistry·Iain B H Wilson, Katharina Paschinger
Nov 2, 2015·Applied Microbiology and Biotechnology·Jerica SabotičMarkus Künzler
Aug 19, 2015·Parasites & Vectors·Christian HeimSaša Štefanić
Oct 22, 2016·Biochimica Et Biophysica Acta. General Subjects·Carmen Jiménez-CastellsKatharina Paschinger
Nov 13, 2015·PLoS Pathogens·Joao A RodriguesLuis Izquierdo
May 24, 2015·Molecular & Cellular Proteomics : MCP·Shi YanKatharina Paschinger
Jan 13, 2018·Glycobiology·Ewa JankowskaJohn F Cipollo
May 7, 2019·Parasitology·Carolina De Marco VeríssimoAlessandra L Morassutti
Jul 7, 2019·Glycoconjugate Journal·Katharina Paschinger, Iain B H Wilson
Jul 11, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Alexandra Martins Dos Santos SoaresLivio Martins Costa Junior
May 3, 2019·Parasitology·Katharina Paschinger, Iain B H Wilson
Mar 28, 2019·Frontiers in Molecular Biosciences·Katharina PaschingerIain B H Wilson
Jan 10, 2019·Nature Communications·Francesca MartiniKatharina Paschinger
May 25, 2021·Computational and Structural Biotechnology Journal·Chunqun WangMin Hu
Dec 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Charlotte ToustouMuriel Bardor

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