PMID: 9531501Jun 11, 1998Paper

Two types of H+-ATPase are involved in the acidification of internal compartments in Trypanosoma cruzi

The Biochemical Journal
D A Scott, R Docampo

Abstract

ATP-driven acidification of internal compartments of Trypanosoma cruzi epimastigotes was assayed spectrophotometrically with Acridine Orange and cells permeabilized with filipin. H+-ATPase activity was not inhibited fully by either 500 nM concanamycin A or 500 microM orthovanadate, but a combination of 5 nM concanamycin A and 25 microM vanadate completely inhibited activity, suggesting the operation of separate V-type (concanamycin-sensitive) and P-type (vanadate-sensitive) H+-ATPase activities in the permeabilized cells. This was supported by different kinetics of Acridine Orange uptake seen in the presence of the different inhibitors, and by different optimal protein (cell) concentrations for the two apparent activities. The use of different buffers further distinguished the ATPases. The V-H+-ATPase activity was stimulated by K+ and inhibited by a lack of anions or the replacement of Cl- with gluconate. The P-type H+-ATPase activity was not affected by a lack of Cl- or K+ but was substantially inhibited in a largely anion-free buffer. This inhibition could be annulled by the addition of the K+ ionophore valinomycin, which probably acted via the establishment of a countercurrent efflux of K+ from the compartment containing the...Continue Reading

Citations

Dec 30, 2008·Insect Biochemistry and Molecular Biology·Lucimar S MottaEdnildo A Machado
Mar 12, 2005·Molecular and Biochemical Parasitology·Lia Carolina A Soares MedeirosHector Barrabin
Aug 3, 2010·Biochimica Et Biophysica Acta·Laurent BonneauFrançoise Simon-Plas
May 24, 2003·Memórias do Instituto Oswaldo Cruz·Wanderley de Souza, Narcisa Leal da Cunha-e-Silva
Feb 11, 2005·The Journal of Eukaryotic Microbiology·Kildare MirandaRoberto Docampo
Nov 30, 2019·Parasite : Journal De La Société Française De Parasitologie·John C Meade
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
Jun 8, 2006·The Journal of Biological Chemistry·Shuhong LuoRoberto Docampo
Jun 21, 2018·Genes·Srinivasan Ramakrishnan, Roberto Docampo
May 19, 2000·The Journal of Biological Chemistry·D A Scott, R Docampo
Sep 20, 2008·The Journal of Biological Chemistry·Alejandra C SchoijetGuillermo D Alonso
Aug 15, 1998·The Journal of Biological Chemistry·D A ScottR Docampo
Jan 30, 2002·Molecular and Biochemical Parasitology·Norma Marchesini, Roberto Docampo

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