PMID: 9557690Apr 29, 1998Paper

Two types of virus-related particles are found during transmissible gastroenteritis virus morphogenesis

Journal of Virology
Cristina RiscoJosé L Carrascosa

Abstract

The intracellular assembly of the transmissible gastroenteritis coronavirus (TGEV) was studied in infected swine testis (ST) cells at different postinfection times by using ultrathin sections of conventionally embedded infected cells, freeze-substitution, and methods for detecting viral proteins and RNA at the electron microscopy level. This ultrastructural analysis was focused on the identification of the different viral components that assemble in infected cells, in particular the spherical, potentially icosahedral internal core, a new structural element of the extracellular infectious coronavirus recently characterized by our group. Typical budding profiles and two types of virion-related particles were detected in TGEV-infected cells. While large virions with an electron-dense internal periphery and a clear central area are abundant at perinuclear regions, smaller viral particles, with the characteristic morphology of extracellular virions (exhibiting compact internal cores with polygonal contours) accumulate inside secretory vesicles that reach the plasma membrane. The two types of virions coexist in the Golgi complex of infected ST cells. In nocodazole-treated infected cells, the two types of virions coexist in altered Go...Continue Reading

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Citations

Dec 28, 2002·Journal of Virology·Iñigo J SalanuevaCristina Risco
Mar 29, 2003·Journal of Virology·Lili Kuo, Paul S Masters
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Montserrat BárcenaBerend Jan Bosch
May 23, 2006·Annual Review of Microbiology·Luis EnjuanesSonia Zuñiga
Sep 11, 1999·Journal of Virology·I J SalanuevaC Risco
Apr 13, 2001·Microscopy Research and Technique·C QuintanaJ L Carrascosa

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