Two unique recombinant forms identified in incident HIV type 1 infections in Thai blood donors

AIDS Research and Human Retroviruses
Wiriya RutvisuttinuntSodsai Tovanabutra

Abstract

HIV-1 genetic diversity of recently seroconverting (<12 months) Thai repeated blood donors attending the National Blood Centre, Thai Red Cross Society (NBC, TRCS) from September 2007 until March 2008 was assessed. Ten HIV-1 recent seroconvertors (10/239,134 donations) were identified during the study period. The estimated median time to seroconversion was 67.3 days (range: 45.5-102.0 days), and viral load ranged from 307 to 341,805 copies HIV-1 RNA/ml. MHAbce, a real-time-based PCR genotyping assay, identified six CRF01_AE, two CRF01_AE/B recombinants, one subtype B, and one CRF01_AE/B dual infection. Nine samples were further characterized by full genome sequencing, identifying CRF01_AE (N=6), unique CRF01_AE/B recombinants (N=2), and subtype B (N=1). One recombinant contained 13 breakpoints located in gag, pol, vif, vpr, env, and nef while the other recombinant contained 10 breakpoints located in pol, vif, env, and nef. This study found two unique CRF01B recombinants circulating in 10 recent HIV-1-positive subjects from a blood donor population in Thailand.

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Citations

Nov 5, 2014·AIDS Research and Human Retroviruses·Wanna LeelawiwatMarcel Curlin
Jan 30, 2020·Current HIV Research·Poramed Winichakoon, Siripong Tongjai

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Datasets Mentioned

BETA
JN860761

Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

Vironostika
MacGDE
Prism
Determine
p HMM
Sequencher
SDS
MHAbce
DNADIST
DIVEIN

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