PMID: 8981229Dec 1, 1996Paper

Two variants of the pituitary specific transcription factor Pit-1 in Atlantic salmon

Journal of Molecular Endocrinology
J B LorensR Male

Abstract

We have cloned two 1.6 kb cDNAs encoding variants of the POU-type pituitary-specific transcription factor Pit-1 from Atlantic salmon. Sequence comparison with mammalian Pit-1 revealed that the POU domain was highly similar while flanking regions were less conserved. The N-terminal region contained three insertions relative to mammalian Pit-1, one of these corresponded to the insertion found in the alternatively spliced Pit-1a isoform. While two different salmon Pit-1 transcripts were expressed, alternative splicing in the 5'-region did not appear to contribute to further transcript diversity. Both salmon Pit-1 cDNAs encoded 39.5 kDa proteins that specifically bind a consensus Pit-1 recognition sequence in vitro. The salmon Pit-1 proteins also recognized the classical octamer motif; however, a point mutation in the POU homeodomain abolished this interaction.

Citations

Mar 3, 2011·General and Comparative Endocrinology·Anna Rita AngotziDaniel Chourrout
Apr 26, 2006·Genetica·Estela BastosRobert Renaville
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S HarveyK L Hull
Jan 8, 2000·Acta Paediatrica. Supplement·R W PfäffleH Kentrup

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.