Two waves of neutrophil emigration in response to corneal epithelial abrasion: distinct adhesion molecule requirements

Investigative Ophthalmology & Visual Science
Zhijie LiC Wayne Smith

Abstract

Corneal abrasion results in an inflammatory response characterized by leukocyte emigration into the corneal stroma. Adhesion molecules play a critical role in leukocyte emigration to wound sites, but differences are evident in different vascular beds. In this study, the contributions of two families of adhesion molecules to neutrophil emigration into the cornea were investigated. Re-epithelialization, patterns of neutrophil influx and CXC chemokine production were assessed in C57Bl/6 mice after removal of a 2-mm diameter area of central corneal epithelium. Comparisons were made between wild-type (WT) mice and mice with targeted deletions of genes for CD18 (CD18(-/-)) or P- and E-selectin (P/E-sel(-/-)) or in mice with antibody-induced neutropenia. Wild-type mice exhibited neutrophil emigration in two waves, the first peaking at 18 hours and the second at 30 hours after wounding, 6 hours after epithelial wound closure and peak levels of corneal CXCL1. In CD18(-/-) animals, only a single wave of neutrophil influx was seen, and it was temporally and quantitatively equivalent to the second wave in WT. In P/E-sel(-/-) mice, neutrophil influx was markedly depressed throughout the 48-hour observation period. Re-epithelialization was s...Continue Reading

Citations

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