TXNIP mediated the oxidative stress response in glomerular mesangial cells partially through AMPK pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Wenwei XuYaoming Xue

Abstract

Thioredoxin-interacting protein (TXNIP) plays an important role in the development of diabetic nephropathy. In the present study, we investigated role of TXNIP on oxidative stress in glomerular mesangial cells (GMCs) cultured in high glucose or normal glucose, and explored the potential mechanism related to TXNIP as well. Oxidative stress in GMCs under high or normal glucose was detected. TXNIP knockdown by specific siRNA or over expression by pcDNA3.0-TXNIP vector was performed to evaluate the role of TXNIP on injury of GMCs caused by oxidative stress. Activator of AMPK AICAR and AMPK inhibitor Compound C were treated the GMCs. Reactive oxygen species (ROS) and mitochondrial membrane potential were detected by flow cytometry. Activities of superoxide dismutase (SOD) and superoxide dismutase (CAT) were measured by ELISA. Activity of thioredoxin (Trx) was determined using Trx activity assay kit. mRNA expression of AMPK, TXNIP, Trx1 and Trx2 were tested by qRT-PCR. Expressions of P-AMPK, TXNIP and fibronectin proteins were detected by Western blotting. High glucose induced the increase of ROS level, activation of TXNIP, but restricted mitochondrial membrane potential and activities of p-AMPK, SOD and CAT, and Trx. TXNIP siRNA and...Continue Reading

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Citations

Mar 5, 2020·Proteomics·Venkateswaran NatarajanKarthik Mallilankaraman
Dec 20, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hai HuShengqing Yu
Mar 17, 2020·Frontiers in Cell and Developmental Biology·Huiling XiangJing Xiong
Feb 12, 2021·International Journal of Molecular Sciences·Alison DominguesValérie Nivet-Antoine
Dec 5, 2020·Oxidative Medicine and Cellular Longevity·Xiaoqiang XiaoHaoyu Chen
Jun 4, 2021·Journal of Inflammation Research·Abdullah Al MamunJian Xiao

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