Type 1 diabetes mellitus induces structural changes and molecular remodelling in the rat kidney

Molecular and Cellular Biochemistry
Raphael M SinghTehreem Waqar

Abstract

There is much evidence that diabetes mellitus (DM)-induced hyperglycemia (HG) is responsible for kidney failure or nephropathy leading to cardiovascular complications. Cellular and molecular mechanism(s) whereby DM can damage the kidney is still not fully understood. This study investigated the effect of streptozotocin (STZ)-induced diabetes (T1DM) on the structure and associated molecular alterations of the isolated rat left kidney following 2 and 4 months of the disorder compared to the respective age-matched controls. The results revealed hypertrophy and general disorganized architecture of the kidney characterized by expansion in glomerular borders, tubular atrophy and increased vacuolization of renal tubular epithelial cells in the diabetic groups compared to controls. Electron microscopic analysis revealed ultrastructural alterations in the left kidney highlighted by an increase in glomerular basement membrane width. In addition, increased caspase-3 immunoreactivity was observed in the kidney of T1DM animals compared to age-matched controls. These structural changes were associated with elevated extracellular matrix (ECM) deposition and consequently, altered gene expression profile of ECM key components, together with ele...Continue Reading

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Citations

May 2, 2019·Experimental Biology and Medicine·Cláudia Silva SouzaTelma de Jesus Soares
Jun 4, 2020·Experimental Biology and Medicine·Liliany Souza de Brito AmaralTelma de Jesus Soares
Mar 9, 2019·Environmental Science and Pollution Research International·Marcela Nascimento SertorioMariana Machado-Neves

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Methods Mentioned

BETA
light microscopy
PCR
protein
Enzyme-Linked
enzyme-linked immunosorbent assay

Software Mentioned

ELIZA
SPSS
SDS
Image J

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