Type-1 insulin-like growth factor receptor reexpression in the malignant phenotype of SV40-T-immortalized human prostate epithelial cells enhances apoptosis

Endocrine
Stephen R PlymateJoy L Ware

Abstract

The authors have previously shown that the type 1 insulin-like growth factor receptor (IGF-1R) is decreased in the transformation from benign to malignant human prostate epithelial cells in vivo. Further, in a well-described human SV40-T immortalized human epithelial cell system beginning with the immortalized, but rarely tumorigenic P69SV40-T cell line, to the highly tumorigenic and metastatic M12 subline, there is a similar decrease in IGF-1R number from 2.0 x 10(4) receptors per cell to 1.1 x 10(3) receptors per cell. When the IGF-1R was reexpressed in the M12 subline using a retroviral expression vector, M12-LISN, to a receptor number similar to that of the P69SV40-T parental cell line, the authors demonstrated a marked decrease in colony formation in soft agar in the M12-LISN cells vs the M12 control cells (p < or = 0.01), and a decrease in vivo tumor growth and metastases when injected either subcutaneously or an intraprostatic location (p < or = 0.01). This decrease in tumor volume was not because of a decrease in proliferative capacity, but was associated with an increase in apoptosis in baseline cultures and in response to the apoptotic-inducing agents 6-hydroxyurea, retinoic acid, and transforming growth factor beta 1.

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Citations

Jul 25, 2000·Journal of Pediatric Endocrinology & Metabolism : JPEM·C LeeD M Cooper
Jul 9, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·R J BoltH A Delemarre-van de Waal
Jan 16, 2007·Journal of Pediatric Endocrinology & Metabolism : JPEM·Matthias HeckmannStefan A Wudy
Jun 11, 2008·Journal of Pediatric Endocrinology & Metabolism : JPEM·Pen-Hua SuJui-Chen Ke
May 4, 2010·Journal of Pediatric Endocrinology & Metabolism : JPEM·Zvi Zadik
May 4, 2010·Journal of Pediatric Endocrinology & Metabolism : JPEM·Hui WangJiang Yue
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SperandioD E Bredesen
Feb 25, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Katia ScotlandiPiero Picci
Jun 7, 2002·The Journal of Urology·Charles A SeaburyJohn Petros
Apr 28, 2006·Journal of Cellular Biochemistry·Jennifer D WuStephen R Plymate
Jun 15, 2004·Cell Death and Differentiation·S SperandioD E Bredesen
Jun 2, 2012·Endocrinology·María Celia FernándezPatricia A Pennisi

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