Type-2 diabetes-induced changes in vascular extracellular matrix gene expression: relation to vessel size

Cardiovascular Diabetology
WeiWei Song, Adviye Ergul

Abstract

Hyperglycemia-induced changes in vascular wall structure contribute to the pathogenesis of diabetic microvascular and macrovascular complications. Matrix metalloproteinases (MMP), a family of proteolytic enzymes that degrade extracellular matrix (ECM) proteins, are essential for vascular remodeling. We have shown that endothelin-1 (ET-1) mediates increased MMP activity and associated vascular remodeling in Type 2 diabetes. However, the effect of Type 2 diabetes and/or ET-1 on the regulation of ECM and MMP gene expression in different vascular beds remains unknown. Aorta and mesenteric artery samples were isolated from control, Type 2 diabetic Goto-Kakizaki (GK) rats and GK rats treated with ETA antagonist ABT-627. Gene expression profile of MMP-2, MMP-9, MT1-MMP, fibronectin, procollagen type 1, c-fos and c-jun, were determined by quantitative real-time (qRT) PCR. In addition, aortic gene expression profile was evaluated by an ECM & Adhesion Molecules pathway specific microarray approach. Analysis of the qRT-PCR data demonstrated a significant increase in mRNA levels of MMPs and ECM proteins as compared to control animals after 6 weeks of mild diabetes. Furthermore, these changes were comparable in aorta and mesentery samples. ...Continue Reading

References

Mar 1, 1997·The Journal of Clinical Investigation·J R RumbleR E Gilbert
Jan 12, 1999·Microvascular Research·D VranesR J Dilley
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jul 5, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Adviye ErgulLeslie C Fuchs
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Paul BornsteinThemis R Kyriakides
Sep 15, 2004·American Journal of Hypertension·Adviye ErgulMark P Anstadt
Dec 8, 2004·Diabetes/metabolism Research and Reviews·Gen FukudaSubrata Chakrabarti
Oct 22, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Vera Portik-DobosAdviye Ergul
Feb 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer C SullivanJennifer S Pollock

❮ Previous
Next ❯

Citations

Jan 10, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Brittany LawEdie C Goldsmith
Mar 13, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Wentong CuiDali Luo
Jun 3, 2008·Canadian Journal of Physiology and Pharmacology·Mostafa M ElgebalyAdviye Ergul
Jul 16, 2010·Canadian Journal of Physiology and Pharmacology·Kamakshi SachidanandamAdviye Ergul
Mar 8, 2017·Biology of the Cell·Caroline Arous, Bernhard Wehrle-Haller
Oct 7, 2008·The Annals of Otology, Rhinology, and Laryngology·Tsunehisa OhnoBernard Rousseau
Sep 8, 2009·Journal of Cardiovascular Pharmacology and Therapeutics·Buket ReelZeliha Kerry
Sep 23, 2008·Annals of Biomedical Engineering·Alejandro J AlmarzaSavio L-Y Woo
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gregory S Schultz, Annette Wysocki
Mar 5, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dongrim SeolJames A Martin
Mar 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Joseph CandelaCarl White
Aug 16, 2008·Biological Trace Element Research·Aslihan Aydemir-Koksoy, Belma Turan
Jun 10, 2009·The Laryngoscope·Tsunehisa OhnoBernard Rousseau
Feb 17, 2021·Thrombosis and Haemostasis·Marisol Herrera-RiveroUlrike Nowak-Göttl
Mar 27, 2021·Molecular Therapy. Nucleic Acids·Isabel N SchellingerUwe Raaz
Jan 24, 2012·The Journal of Thoracic and Cardiovascular Surgery·Yongxin SunChunsheng Wang
Jul 6, 2010·Experimental Cell Research·Soon-Mi LimAndreea Trache

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

SAM ( Statistical Analysis of Microarrays )
GEArray Analyzer
ScanAlyze

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.