Type 2 Diabetes Promotes Cell Centrosome Amplification via AKT-ROS-Dependent Signalling of ROCK1 and 14-3-3σ

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Pu WangShaochin Lee

Abstract

Type 2 diabetes is associated with oxidative stress and DNA damage which can cause centrosome amplification. Thus, the study investigated centrosome amplification in type 2 diabetes and the underlying mechanisms. Centrosome numbers in human peripheral blood mononuclear blood cells (PBMC) from healthy subjects and patients with type 2 diabetes were compared to access the association between type 2 diabetes and centrosome amplification. Colon cancer cells were used to investigate the molecular mechanisms underlying the centrosome amplification triggered by high glucose, insulin and palmitic acid. Western blot analysis was used to quantify the level of protein and protein phosphorylation. Immunofluorescent staining was performed to detect centrosomes. ROS was quantified using flow cytometry technique. Transcriptpmic profiling was performed using Illumina HiSeqTM500 platform. We found that centrosome amplification was increased PBMC from the type 2 diabetic patients, which correlated with the levels of fasting blood glucose and HbA1c. High glucose, insulin and palmitic acid, alone or in combinations, induced ROS production and centrosome amplification. Together, they increased AKT activation as well as the expression, binding and c...Continue Reading

Citations

May 24, 2019·Free Radical Research·David L NewmanStephen L Gregory
Sep 22, 2019·International Journal of Molecular Sciences·David L Newman, Stephen L Gregory
Aug 1, 2020·Nucleic Acids Research·Gal Barel, Ralf Herwig
Oct 17, 2019·American Journal of Physiology. Cell Physiology·Qin Ju HeShao Chin Lee
Jan 2, 2021·The Journal of Investigative Dermatology·Matthew T PatrickLam C Tsoi
May 17, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Ji Zhong ZhaoShao Chin Lee

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