Type 3 inositol 1,4,5-trisphosphate receptor has antiapoptotic and proliferative role in cancer cells

Cell Death & Disease
Ingeborg RezuchovaOlga Krizanova

Abstract

Although the involvement of type 1 (IP3R1) and type 2 (IP3R2) inositol 1,4,5-trisphosphate receptors in apoptosis induction has been well documented in different cancer cells and tissues, the function of type 3 IP3R (IP3R3) is still elusive. Therefore, in this work we focused on the role of IP3R3 in tumor cells in vitro and in vivo. We determined increased expression of this receptor in clear cell renal cell carcinoma compared to matched unaffected part of the kidney from the same patient. Thus, we hypothesized about different functions of IP3R3 compared to IP3R1 and IP3R2 in tumor cells. Silencing of IP3R1 prevented apoptosis induction in colorectal cancer DLD1 cells, ovarian cancer A2780 cells, and clear cell renal cell carcinoma RCC4 cells, compared to apoptosis in cells treated with scrambled siRNA. As expected, silencing of IP3R3 and subsequent apoptosis induction resulted in increased levels of apoptosis in all these cells. Further, we prepared a DLD1/IP3R3_del cell line using CRISPR/Cas9 gene editing method. These cells were injected into nude mice and tumor's volume was compared with tumors induced by DLD1 cells. Lower volume of tumors originated from DLD1/IP3R3_del cells was observed after 12 days, compared to wild typ...Continue Reading

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Citations

Jul 23, 2020·Journal of Cellular Physiology·Hyocheol BaeWhasun Lim
Sep 20, 2020·Annals of Clinical and Translational Neurology·Julius RönkköEmil Ylikallio
Apr 16, 2020·Journal of Clinical Medicine·Jessica GambardellaGaetano Santulli
Aug 14, 2020·Cancers·Laura CaraviaSilviu Cristian Voinea
Sep 13, 2020·International Journal of Molecular Sciences·Carlos Pascual-CaroFrancisco Javier Martin-Romero
Jul 25, 2020·Journal of Hematology & Oncology·Chaeuk ChungEun-Kyeong Jo
Jul 22, 2020·Cancer Discovery·Chengheng LiaoQing Zhang
Jan 6, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Yufeng WuQingyi Wei
Dec 15, 2020·Briefings in Bioinformatics·Jingbo YangXiujie Chen
Nov 12, 2020·International Journal of Molecular Sciences·Simone PatergnaniCarlotta Giorgi
Feb 13, 2021·Journal of Experimental & Clinical Cancer Research : CR·Mengzhao ZhangJinhai Fan
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Aurora Gil-HernándezAlejandro Silva-Palacios
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Ulises Ahumada-CastroCésar Cárdenas
Aug 7, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Young Min KimHyojin Park
Jul 20, 2021·Physiological Reviews·Kellie A Woll, Filip Van Petegem
Oct 21, 2021·The FEBS Journal·Robert E Means, Samuel G Katz

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Methods Mentioned

BETA
xenograft
transfection
protein knockout
electrophoresis
proximity ligation assay
xenografts

Software Mentioned

GraphPad
IncuCyte [UNK]
Leica Application
InStat
LAS AF

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